caa a22 4500 467944504 CHVBK 20180405193735.0 cr unu---uuuuu 170328e20041001xx s 000 0 eng 10.1023/B:GROW.0000049411.54953.b4 doi (NATIONALLICENCE)springer-10.1023/B:GROW.0000049411.54953.b4 (NATIONALLICENCE)springer-10.1007/s10725-004-2811-0 Effects of light conditions and 2,4-D concentration in soybean anther culture [Elektronische Daten] [L.R. Rodrigues, B. de Forte, J.M.S. Oliveira, J.E.A. Mariath, M.H. Bodanese-Zanettini] The morphogenic response of anther walls and connective tissue is the greatest obstacle to androgenesis in soybean anther culture. Whereas induction to microspore embryogenesis occurs in the dark in almost all plant species, soybean anthers have been cultured under light. In an attempt to establish culture conditions that simultaneously stimulate microspore embryogenesis and inhibit epidermal and connective cell proliferation, the effect of light and two 2,4-dichlorophenoxyacetic acid (2,4-D) concentrations (2 and 10 mg l−1) on the induction process was investigated. Higher 2,4-D concentration speeded up microspore plasmolysis and did not improve androgenesis. Callogenesis and embryogenesis induction from sporophytic cells were significantly lower in the dark, and some microspores showed major alterations in the sporoderm. Auxin 2,4-D and induction under light contributed to the morphogenic response of the anther walls and connective tissue under the conditions previously recommended to trigger microspore embryogenesis. Kluwer Academic Publishers, 2004 Androgenesis nationallicence Culture conditions nationallicence 2,4-D nationallicence Glycine max nationallicence Light nationallicence Somatic embryogenesis nationallicence Rodrigues L.R. Departamento de Genética, Universidade Federal do Rio Grande do Sul, P.O. Box, 15053, CEP 91501-970, Porto Alegre, RS, Brazil aut de Forte B. Departamento de Genética, Universidade Federal do Rio Grande do Sul, P.O. Box, 15053, CEP 91501-970, Porto Alegre, RS, Brazil aut Oliveira J.M.S. Departamento de Botânica, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil aut Mariath J.E.A. Departamento de Botânica, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil aut Bodanese-Zanettini M.H. Departamento de Genética, Universidade Federal do Rio Grande do Sul, P.O. Box, 15053, CEP 91501-970, Porto Alegre, RS, Brazil aut Plant Growth Regulation Kluwer Academic Publishers 44/2(2004-10-01), 125-131 0167-6903 44:2<125 2004 44 10725 https://doi.org/10.1023/B:GROW.0000049411.54953.b4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/B:GROW.0000049411.54953.b4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rodrigues L.R. Departamento de Genética, Universidade Federal do Rio Grande do Sul, P.O. Box, 15053, CEP 91501-970, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- de Forte B. Departamento de Genética, Universidade Federal do Rio Grande do Sul, P.O. Box, 15053, CEP 91501-970, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- Bodanese-Zanettini M.H. Departamento de Genética, Universidade Federal do Rio Grande do Sul, P.O. Box, 15053, CEP 91501-970, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- Oliveira J.M.S. Departamento de Botânica, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- Mariath J.E.A. Departamento de Botânica, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil aut NATIONALLICENCE 773 0- Plant Growth Regulation Kluwer Academic Publishers 44/2(2004-10-01), 125-131 0167-6903 44:2<125 2004 44 10725 NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-004-2811-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rodrigues L.R. Departamento de Genética, Universidade Federal do Rio Grande do Sul, P.O. Box 15053, CEP 91501-970, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- Bodanese-Zanettini M.H. Departamento de Genética, Universidade Federal do Rio Grande do Sul, P.O. Box 15053, CEP 91501-970, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- Forte B. de C. Departamento de Genética, Universidade Federal do Rio Grande do Sul, P.O. Box 15053, CEP 91501-970, Porto Alegre, RS, Brazil aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer