caa a22 4500 467944539 CHVBK 20180405193735.0 cr unu---uuuuu 170328e20050201xx s 000 0 eng 10.1007/s11055-005-0011-7 doi (NATIONALLICENCE)springer-10.1007/s11055-005-0011-7 (NATIONALLICENCE)springer-10.1007/s11055-005-0066-5 Effects of the C-terminal Peptide of the αs subunit of the G protein on the regulation of Adenylyl Cyclase and Protein Kinase A activities by Biogenic Amines and Glucagon in Mollusk and Rat Muscles [Elektronische Daten] [A. Shpakov, V. Korol'kov, S. Plesneva, L. Kuznetsova, M. Pertseva] The C-terminal parts of the α subunits of heteromeric G proteins play an important role in the functional linkage of G proteins with receptors of the serpentine type. The present report describes studies of the effects of the C-terminal octapeptide 387-394 of the αs subunit of the mammalian G protein on the transmission of the hormonal signal via the hormone-sensitive adenylyl cyclase signal system, whose major components are receptors of the serpentine type, G proteins, and the enzymes adenylyl cyclase and protein kinase A. The peptide synthesized here, 387-394 amide (10-7 - 10-4 M), dose-dependently decreased adenylyl cyclase and protein kinase A activities stimulated by serotonin and glucagon in smooth muscle from the freshwater bivalve mollusk Anodonta cygnea and by the β agonist isoproterenol in rat skeletal muscle. At a concentration as low as 10-7 M, the peptide released potentiation of the stimulatory effects of hormones on adenylyl cyclase activity due to the non-hydrolyzable guanine nucleotide analog Gpp[NH]p. At the same time, it had almost no effect on the stimulation of adenylyl cyclase activity by non-hormonal agents (NaF, Gpp[NH]p, and forskolin). The inhibitory effects of hormones on adenylyl cyclase and protein kinase A activities persisted in the presence of the peptide. Our data demonstrate the importance of the C-terminal part of the αs subunit of the stimulatory G protein for its functional linkage with receptors of the serpentine type and throw light on the molecular mechanisms of the interactions between G proteins and receptors. Springer Science+Business Media, Inc., 2005 adenylyl cyclase nationallicence G protein α subunits nationallicence glucagon nationallicence isoproterenol nationallicence the mollusk Anodonta cygnea nationallicence protein kinase A nationallicence serpentine-type receptors nationallicence C-terminal peptide nationallicence serotonin nationallicence Shpakov A. I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, 44 M. Torez Prospekt, 194223, St. Petersburg, Russia aut Korol'kov V. I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, 44 M. Torez Prospekt, 194223, St. Petersburg, Russia aut Plesneva S. I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, 44 M. Torez Prospekt, 194223, St. Petersburg, Russia aut Kuznetsova L. I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, 44 M. Torez Prospekt, 194223, St. Petersburg, Russia aut Pertseva M. I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, 44 M. Torez Prospekt, 194223, St. Petersburg, Russia aut Neuroscience and Behavioral Physiology Kluwer Academic Publishers-Consultants Bureau 35/2(2005-02-01), 177-186 0097-0549 35:2<177 2005 35 11055 https://doi.org/10.1007/s11055-005-0011-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s11055-005-0011-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shpakov A. I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, 44 M. Torez Prospekt, 194223, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Korol'kov V. I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, 44 M. Torez Prospekt, 194223, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Plesneva S. I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, 44 M. Torez Prospekt, 194223, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Kuznetsova L. I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, 44 M. Torez Prospekt, 194223, St. Petersburg, Russia aut NATIONALLICENCE 700 1- Pertseva M. I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, 44 M. Torez Prospekt, 194223, St. Petersburg, Russia aut NATIONALLICENCE 773 0- Neuroscience and Behavioral Physiology Kluwer Academic Publishers-Consultants Bureau 35/2(2005-02-01), 177-186 0097-0549 35:2<177 2005 35 11055 NATIONALLICENCE 856 40 https://doi.org/10.1007/s11055-005-0066-5 text/html Onlinezugriff via DOI Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer