caa a22 4500 467944873 CHVBK 20180323165659.0 cr unu---uuuuu 170328e19880901xx s 000 0 eng 10.1007/BF01982896 doi (NATIONALLICENCE)springer-10.1007/BF01982896 (NATIONALLICENCE)springer-10.1007/BF00939301 Alcohol production from glucose and xylose using Escherichia coli containing Zymomonas mobilis genes [Elektronische Daten] [A. Neale, R. Scopes, J. Kelly] Summary: AnEscherichia coli strain containing a recombinant plasmid encoding the pyruvate decarboxylase and alcohol dehydrogenase genes fromZymomonas mobilis metabolized glucose and xylose to near theoretical yields of ethanol. Enzyme activity measurements indicate high expression levels of both plasmid-encodedZymomonas proteins in the recombinantE. coli. The expression inE. coli is under the control of a promoter in theZymomonas sequence upstream of the pyruvate decarboxylase gene. The maximum ethanol level, using 4% glucose as substrate, was 1.8% (w/v) in anaerobic conditions. In aerobic conditions the natural repression ofE. coli alcohol dehydrogenase results in less ethanol production from clones expressing onlyZymomonas pyruvate decarboxylase. Springer-Verlag, 1988 Neale A. Department of Biochemistry, La Trobe University, 3083, Bundoora, Victoria, Australia aut Scopes R. Department of Biochemistry, La Trobe University, 3083, Bundoora, Victoria, Australia aut Kelly J. Department of Biochemistry, La Trobe University, 3083, Bundoora, Victoria, Australia aut Applied Microbiology and Biotechnology Springer-Verlag 29/2-3(1988-09-01), 162-167 0175-7598 29:2-3<162 1988 29 253 https://doi.org/10.1007/BF01982896 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01982896 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Neale A. Department of Biochemistry, La Trobe University, 3083, Bundoora, Victoria, Australia aut NATIONALLICENCE 700 1- Scopes R. Department of Biochemistry, La Trobe University, 3083, Bundoora, Victoria, Australia aut NATIONALLICENCE 700 1- Kelly J. Department of Biochemistry, La Trobe University, 3083, Bundoora, Victoria, Australia aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 29/2-3(1988-09-01), 162-167 0175-7598 29:2-3<162 1988 29 253 NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00939301 text/html Onlinezugriff via DOI Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer