caa a22 4500 467945748 CHVBK 20180323165700.0 cr unu---uuuuu 170328e19891201xx s 000 0 eng 10.1007/BF02396062 doi (NATIONALLICENCE)springer-10.1007/BF02396062 (NATIONALLICENCE)springer-10.1007/BF00553991 Four tandem defective P elements associated with positive regulation of the Drosophila melanogaster glucose-6-phosphate dehydrogenase gene [Elektronische Daten] [Masanobu Itoh, Mari Iwabuchi, Kiyohito Yoshida, Samuel Hori] Three high-glucose-6-phosphate dehydrogenase (G6PD)-activity mutants (2512H, S44H, and 1FH) are characterized by two insertion sequences associated with the G6PD locus; one (Ins1; 3.5 kb long in 2512H and S44H and 2.9 kb long in 1FH) is present just 5′ to exon I and consists of a KP′ (the 32nd base of the KP was replaced by guanine), a core sequence and a KP, and the other is 4.2 kb long and resides within an intron. Southern blot analyses of revertants showing low G6PD activity suggested that the insertion sequence responsible for high G6PD activity may be the core sequence but not the flanking KP and KP′ or the Ins2. DNA sequencing data of the clone carrying the core sequence of 2512H demonstrated that the core sequence is another type of defective P elements (core P). Interestingly, a protein(s) was found in the nuclear extract of Canton S embryos that specifically binds to the core P but not to the KP or various fragments of pπ 25.1. In addition, the mutant G6PD activity was found to be affected not only by the genotype, but also by cytoplasmic factors. Plenum Publishing Corporation, 1989 Drosophila glucose-6-phosphate dehydrogenase (G6PD) gene nationallicence transposable element nationallicence P element nationallicence overproduction mutation nationallicence Itoh Masanobu Department of Zoology, Faculty of Science, Hokkaido University, 060, Sapporo, Japan aut Iwabuchi Mari Department of Zoology, Faculty of Science, Hokkaido University, 060, Sapporo, Japan aut Yoshida Kiyohito Department of Zoology, Faculty of Science, Hokkaido University, 060, Sapporo, Japan aut Hori Samuel Department of Zoology, Faculty of Science, Hokkaido University, 060, Sapporo, Japan aut Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 27/11-12(1989-12-01), 699-718 0006-2928 27:11-12<699 1989 27 10528 https://doi.org/10.1007/BF02396062 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02396062 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Itoh Masanobu Department of Zoology, Faculty of Science, Hokkaido University, 060, Sapporo, Japan aut NATIONALLICENCE 700 1- Iwabuchi Mari Department of Zoology, Faculty of Science, Hokkaido University, 060, Sapporo, Japan aut NATIONALLICENCE 700 1- Yoshida Kiyohito Department of Zoology, Faculty of Science, Hokkaido University, 060, Sapporo, Japan aut NATIONALLICENCE 700 1- Hori Samuel Department of Zoology, Faculty of Science, Hokkaido University, 060, Sapporo, Japan aut NATIONALLICENCE 773 0- Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 27/11-12(1989-12-01), 699-718 0006-2928 27:11-12<699 1989 27 10528 NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00553991 text/html Onlinezugriff via DOI Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer