caa a22 4500 467945829 CHVBK 20180323165700.0 cr unu---uuuuu 170328e19891201xx s 000 0 eng 10.1007/BF00553992 doi (NATIONALLICENCE)springer-10.1007/BF00553992 (NATIONALLICENCE)springer-10.1007/BF02396063 Gene regulation during anaerobiosis in soya roots [Elektronische Daten] [Karoly Tihanyi, Alexis Fontanell, Jean-Paul Thirion] The proteins of soybean roots undergoing anaerobiosis can be grouped into three classes. Class 1 proteins are induced severalfold and at least 28 of these were identified byin vivo labeling. These proteins include the enzymes alcohol dehydrogenase (ADH), fructose aldolase, pyruvate decarboxylase, phosphoglucomutase, and lactate dehydrogenase. Class 2 proteins include such enzymes as glucose phosphate isomerase, sucrase, and malate dehydrogenase; their specific activity remains constant in aerobiosis or anaerobiosis. The third class of proteins includes those enzymes such as peroxidase whose activity decreases more than 90% after just 1 day in anaerobiosis. Immunoblotting coupled with two-dimensional chromatography ofin vitro translated plant extracts demonstrated that ADH level during anaerobiosis is controlled by its mRNA concentration. Little or no mRNA for ADH was detected in aerobically grown roots. This suggests that the increased level of ADH activity is due to de novo synthesis of the mRNA rather than activation of a sequestered mRNA or superactivation of the protein. Plenum Publishing Corporation, 1989 soya nationallicence anaerobiosis nationallicence alcohol dehydrogenase nationallicence lactate dehydrogenase nationallicence fructose aldolase nationallicence phosphoglucomutase nationallicence pyruvate decarboxylase nationallicence peroxidase nationallicence glucose phosphate isomerase nationallicence sucrase nationallicence malate dehydrogenase nationallicence Tihanyi Karoly Department of Microbiology, University of Sherbrooke, J1H 5N4, Sherbrooke, Que, Canada aut Fontanell Alexis Department of Microbiology, University of Sherbrooke, J1H 5N4, Sherbrooke, Que, Canada aut Thirion Jean-Paul Department of Microbiology, University of Sherbrooke, J1H 5N4, Sherbrooke, Que, Canada aut Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 27/11-12(1989-12-01), 719-730 0006-2928 27:11-12<719 1989 27 10528 https://doi.org/10.1007/BF00553992 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00553992 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tihanyi Karoly Department of Microbiology, University of Sherbrooke, J1H 5N4, Sherbrooke, Que, Canada aut NATIONALLICENCE 700 1- Fontanell Alexis Department of Microbiology, University of Sherbrooke, J1H 5N4, Sherbrooke, Que, Canada aut NATIONALLICENCE 700 1- Thirion Jean-Paul Department of Microbiology, University of Sherbrooke, J1H 5N4, Sherbrooke, Que, Canada aut NATIONALLICENCE 773 0- Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 27/11-12(1989-12-01), 719-730 0006-2928 27:11-12<719 1989 27 10528 NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02396063 text/html Onlinezugriff via DOI Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer