caa a22 4500 467945926 CHVBK 20180323165700.0 cr unu---uuuuu 170328e19881001xx s 000 0 eng 10.1007/PL00020502 doi (NATIONALLICENCE)springer-10.1007/PL00020502 (NATIONALLICENCE)springer-10.1007/BF02399607 Genetic control of ganglioside biosynthesis in mice [Elektronische Daten] [Dina Sokoloff, Peter Hechtman] The enzymatic basis for the differences in hepatic ganglioside patterns in the mouse strains C57Bl/6 and Swiss White (SW) was investigated. SW has a "Swiss-type” ganglioside profile, expressing GM1 − and GD1a − in addition to GM2 − as major hepatic gangliosides, whereas C57Bl/6 shows a "GM2-type” profile, expressing only GM2 − as the major hepatic ganglioside. The enzyme UDP-galactose:GM2 ganglioside galactosyltransferase (GM2-GalT), which catalyzes the synthesis of GM1 ganglioside, showed a four- to fivefold elevation in intact and solubilized liver Golgi membrane fractions of the SW strain compared to C57Bl/6. Crosses between C57Bl/6 and SW produced an F1 generation with a hepatic ganglioside and enzymatic phenotype intermediate between those of the two parental strains. All three genotypic groups show two forms of the Golgi apparatus enzyme with isoelectric points of 6.5-6.8 and 8.3-9.0. The simplest mode of action of genes which control the enzymatic phenotype that would be consistent with these findings are one or two structural genes or one or two cis-regulatory genes affecting the rate of enzyme synthesis. Plenum Publishing Corporation, 1988 ganglioside biosynthesis nationallicence galactosyltransferase nationallicence Golgi apparatus nationallicence Sokoloff Dina Centre for Human Genetics, Department of Biology, McGill University, H3A 2T6, Montreal, Quebec, Canada aut Hechtman Peter Centre for Human Genetics, Department of Biology, McGill University, H3A 2T6, Montreal, Quebec, Canada aut Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 26/9-10(1988-10-01), 631-644 0006-2928 26:9-10<631 1988 26 10528 https://doi.org/10.1007/PL00020502 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00020502 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sokoloff Dina Centre for Human Genetics, Department of Biology, McGill University, H3A 2T6, Montreal, Quebec, Canada aut NATIONALLICENCE 700 1- Hechtman Peter Centre for Human Genetics, Department of Biology, McGill University, H3A 2T6, Montreal, Quebec, Canada aut NATIONALLICENCE 773 0- Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 26/9-10(1988-10-01), 631-644 0006-2928 26:9-10<631 1988 26 10528 NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02399607 text/html Onlinezugriff via DOI Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer