caa a22 4500 467945977 CHVBK 20180323165700.0 cr unu---uuuuu 170328e19881201xx s 000 0 eng 10.1007/BF00553871 doi (NATIONALLICENCE)springer-10.1007/BF00553871 (NATIONALLICENCE)springer-10.1007/BF02395518 Altered expression of chitin synthetase activity and biochemical changes in the cell wall in a developmental mutant of Phycomyces [Elektronische Daten] [Elvia Ruiz-Flores, Everardo Lopez-Romero, Arturo Flores-Carreon, Felix Gutierrez-Corona] ThePhycomyces developmental mutant S356 elaborates spores which show a much poorer viability and a higher affinity for Calcofluor White than the wild-type spores. Protease-activated extracts of the mutant spores showed higher levels of chitin synthetase activity than the parental strain-derived spores. High levels of enzyme activity in the mutant extracts, but not in the corresponding wild-type extracts, could be detected in the absence of an exogenous protease. The high basal active chitin synthetase is not the result of activation by endogeneous proteases during cell breakage since protease inhibitors did not reduce, but rather increased, the activity levels. The analysis of cell wall composition in the mutant spores revealed significant changes in the proportion of uronic acids and protein but not in chitin. The mutant phenotype is discussed in relation to the developmental stage at which the alterations connected with cell wall metabolism occurred. Plenum Publishing Corporation, 1988 Phycomyces spores nationallicence developmental mutant nationallicence Calcofluor nationallicence chitin synthetase nationallicence cell wall nationallicence Ruiz-Flores Elvia Instituto de Investigacion en Biologia Experimental, Facultad de Quimica, Universidad de Guanajuato, Apartado Postal 187, 36000, Guanajuato, Gto, Mexico aut Lopez-Romero Everardo Instituto de Investigacion en Biologia Experimental, Facultad de Quimica, Universidad de Guanajuato, Apartado Postal 187, 36000, Guanajuato, Gto, Mexico aut Flores-Carreon Arturo Instituto de Investigacion en Biologia Experimental, Facultad de Quimica, Universidad de Guanajuato, Apartado Postal 187, 36000, Guanajuato, Gto, Mexico aut Gutierrez-Corona Felix Instituto de Investigacion en Biologia Experimental, Facultad de Quimica, Universidad de Guanajuato, Apartado Postal 187, 36000, Guanajuato, Gto, Mexico aut Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 26/11-12(1988-12-01), 717-732 0006-2928 26:11-12<717 1988 26 10528 https://doi.org/10.1007/BF00553871 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00553871 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ruiz-Flores Elvia Instituto de Investigacion en Biologia Experimental, Facultad de Quimica, Universidad de Guanajuato, Apartado Postal 187, 36000, Guanajuato, Gto, Mexico aut NATIONALLICENCE 700 1- Lopez-Romero Everardo Instituto de Investigacion en Biologia Experimental, Facultad de Quimica, Universidad de Guanajuato, Apartado Postal 187, 36000, Guanajuato, Gto, Mexico aut NATIONALLICENCE 700 1- Flores-Carreon Arturo Instituto de Investigacion en Biologia Experimental, Facultad de Quimica, Universidad de Guanajuato, Apartado Postal 187, 36000, Guanajuato, Gto, Mexico aut NATIONALLICENCE 700 1- Gutierrez-Corona Felix Instituto de Investigacion en Biologia Experimental, Facultad de Quimica, Universidad de Guanajuato, Apartado Postal 187, 36000, Guanajuato, Gto, Mexico aut NATIONALLICENCE 773 0- Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 26/11-12(1988-12-01), 717-732 0006-2928 26:11-12<717 1988 26 10528 NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02395518 text/html Onlinezugriff via DOI Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer