caa a22 4500 467946051 CHVBK 20180323165700.0 cr unu---uuuuu 170328e19880901xx s 000 0 eng 10.1007/BF01982916 doi (NATIONALLICENCE)springer-10.1007/BF01982916 (NATIONALLICENCE)springer-10.1007/BF00939321 Growth of Zymomonas mobilis CP4 on mannitol [Elektronische Daten] [Steven Buchholz, Patrick O'Mullan, Douglas Eveleigh] Summary: The ethanologenZymomonas mobilis has a restricted substrate range, namely glucose, fructose and sucrose. It would be useful to expand its substrate range to include other carbohydrates.Z. mobilis was screened for growth on 30 different carbohydrates and organic acids. A single spontaneous mutant,Z mobilis CP4.60, was isolated which illustrated feeble growth on mannitol as the sole carbohydrate source after three months of incubation. Growth ofZ. mobilis CP4.60 for several months in continuous culture with excess mannitol, and including a round of NTG (N-methyl-N'-nitro-N-nitrosoguanidine) mutagenesis in the chemostat, led to the isolation a sequential series of mutants (CP4.62, CP4.64 and CP4.66), each with improved growth rates on mannitol. Metabolism of mannitol byZ. mobilis is oxygen-dependent, resulting in limited production of ethanol and incresed production of lactic acid. This is an initial example of extension of the substrate range ofZymomonas. The conversion of mannitol to fructose could be via an altered alcohol dehydrogenase. Springer-Verlag, 1988 Buchholz Steven Experimental Station, E228/339, E. I. du Pont de Nemours & Co., 19898, Wilmington, DE, USA aut O'Mullan Patrick Department of Biochemistry, Rutgers University, 08903, New Brunswick, NJ, USA aut Eveleigh Douglas Department of Biochemistry, Rutgers University, 08903, New Brunswick, NJ, USA aut Applied Microbiology and Biotechnology Springer-Verlag 29/2-3(1988-09-01), 275-281 0175-7598 29:2-3<275 1988 29 253 https://doi.org/10.1007/BF01982916 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01982916 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Buchholz Steven Experimental Station, E228/339, E. I. du Pont de Nemours & Co., 19898, Wilmington, DE, USA aut NATIONALLICENCE 700 1- O'Mullan Patrick Department of Biochemistry, Rutgers University, 08903, New Brunswick, NJ, USA aut NATIONALLICENCE 700 1- Eveleigh Douglas Department of Biochemistry, Rutgers University, 08903, New Brunswick, NJ, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 29/2-3(1988-09-01), 275-281 0175-7598 29:2-3<275 1988 29 253 NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00939321 text/html Onlinezugriff via DOI Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer