caa a22 4500 467946337 CHVBK 20180405193737.0 cr unu---uuuuu 170328e20050101xx s 000 0 eng 10.1007/PL00021766 doi (NATIONALLICENCE)springer-10.1007/PL00021766 (NATIONALLICENCE)springer-10.1007/s10541-005-0056-9 Identification of intermediate and product from methemoglobin-catalyzed oxidation of o -phenylenediamine in two-phase aqueous—organic system [Elektronische Daten] [De-Jia Li, Xi-Wen Li, Yu-Xiang Xie, Xiao-Qiang Cai, Guo-Lin Zou] Methemoglobin (metHb) was used as a mimetic enzyme for peroxidase to catalyze the oxidation reaction of o-phenylenediamine (OPDA) with H2O2 functioning as an oxidant. A reaction intermediate was obtained in two-phase aqueous-organic system and an absorption peak at 710 nm was confirmed to be that of the intermediate in relation to OPDA. The isolated product and intermediate were characterized by UV-V is and IR spectrophotometry and HPLC-tandem mass spectrometry. The results showed that the product is 2,3-diaminophenazine, the molecular mass of the intermediate is 212 daltons, and a conceivable structure of the intermediate is suggested. Combining the catalyzed reaction mechanism of peroxidase and our experimental results, a conceivable oxidation reaction mechanism of OPDA and H2O2 using metHb as catalyst is proposed. MAIK "Nauka/Interperiodica”, 2005 hemoglobin nationallicence o -phenylenediamine nationallicence intermediate nationallicence reaction mechanism nationallicence OPDA : o-phenylenediamine nationallicence Hb : hemoglobin nationallicence metHb : methemoglobin nationallicence HRP : horseradish peroxidase nationallicence Li De-Jia College of Life Sciences, Wuhan University, 430072, Wuhan, Hubei, PR China aut Li Xi-Wen College of Life Sciences, Wuhan University, 430072, Wuhan, Hubei, PR China aut Xie Yu-Xiang College of Life Sciences, Wuhan University, 430072, Wuhan, Hubei, PR China aut Cai Xiao-Qiang College of Life Sciences, Wuhan University, 430072, Wuhan, Hubei, PR China aut Zou Guo-Lin College of Life Sciences, Wuhan University, 430072, Wuhan, Hubei, PR China aut Biochemistry (Moscow) Nauka/Interperiodica 70/1(2005-01-01), 92-99 0006-2979 70:1<92 2005 70 10541 https://doi.org/10.1007/PL00021766 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00021766 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li De-Jia College of Life Sciences, Wuhan University, 430072, Wuhan, Hubei, PR China aut NATIONALLICENCE 700 1- Li Xi-Wen College of Life Sciences, Wuhan University, 430072, Wuhan, Hubei, PR China aut NATIONALLICENCE 700 1- Xie Yu-Xiang College of Life Sciences, Wuhan University, 430072, Wuhan, Hubei, PR China aut NATIONALLICENCE 700 1- Cai Xiao-Qiang College of Life Sciences, Wuhan University, 430072, Wuhan, Hubei, PR China aut NATIONALLICENCE 700 1- Zou Guo-Lin College of Life Sciences, Wuhan University, 430072, Wuhan, Hubei, PR China aut NATIONALLICENCE 773 0- Biochemistry (Moscow) Nauka/Interperiodica 70/1(2005-01-01), 92-99 0006-2979 70:1<92 2005 70 10541 NATIONALLICENCE 856 40 https://doi.org/10.1007/s10541-005-0056-9 text/html Onlinezugriff via DOI Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer