caa a22 4500 467946442 CHVBK 20180323165700.0 cr unu---uuuuu 170328e19880901xx s 000 0 eng 10.1007/BF00939296 doi (NATIONALLICENCE)springer-10.1007/BF00939296 (NATIONALLICENCE)springer-10.1007/BF01982891 Improvement in stability of an immobilized fungal laccase [Elektronische Daten] [Andrzej Leonowicz, Jawed Sarkar, Jean-Marc Bollag] Summary: A laccase of the basidiomyceteTrametes versicolor was immobilized on porous glass beads that were activated with 3-aminopropyltriethoxysilane and glutaraldehyde. The support immobilized 100% of the enzyme, whereupon 90% of the original activity was retained. After immobilization, the enzyme was active in a wider pH and temperature range, and its heat stability and reuse were greatly improved compared to those of the free laccase. The immobilized enzyme was found reusable in treating different substrates, either recycled alone or in a sequential order. Springer-Verlag, 1988 Leonowicz Andrzej Laboratory of Soil Biochemistry, Department of Agronomy, The Pennsylvania State University, 16802, University Park, PA, USA aut Sarkar Jawed Laboratory of Soil Biochemistry, Department of Agronomy, The Pennsylvania State University, 16802, University Park, PA, USA aut Bollag Jean-Marc Laboratory of Soil Biochemistry, Department of Agronomy, The Pennsylvania State University, 16802, University Park, PA, USA aut Applied Microbiology and Biotechnology Springer-Verlag 29/2-3(1988-09-01), 129-135 0175-7598 29:2-3<129 1988 29 253 https://doi.org/10.1007/BF00939296 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00939296 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Leonowicz Andrzej Laboratory of Soil Biochemistry, Department of Agronomy, The Pennsylvania State University, 16802, University Park, PA, USA aut NATIONALLICENCE 700 1- Sarkar Jawed Laboratory of Soil Biochemistry, Department of Agronomy, The Pennsylvania State University, 16802, University Park, PA, USA aut NATIONALLICENCE 700 1- Bollag Jean-Marc Laboratory of Soil Biochemistry, Department of Agronomy, The Pennsylvania State University, 16802, University Park, PA, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 29/2-3(1988-09-01), 129-135 0175-7598 29:2-3<129 1988 29 253 NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01982891 text/html Onlinezugriff via DOI Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer