caa a22 4500 467947015 CHVBK 20180323165701.0 cr unu---uuuuu 170328e19891001xx s 000 0 eng 10.1007/BF00553632 doi (NATIONALLICENCE)springer-10.1007/BF00553632 (NATIONALLICENCE)springer-10.1007/BF02396152 Roehrdanz Richard United States Department of Agriculture, Agricultural Research Service, Biosciences Research Laboratory, State University Station, 58105, Fargo, North Dakota aut Intraspecific genetic variability in mitochondrial DNA of the screwworm fly ( Cochliomyia hominivorax ) [Elektronische Daten] [Richard Roehrdanz] Mitochondrial DNA variability has been analyzed in the primary screwworm fly (Cochliomyia hominivorax) using restriction endonuclease fragment patterns and restriction site mapping. A total of 30 different screwworm lines originating from Texas to Costa Rica and the Island of Jamaica was examined using 15 restriction endonucleases. Eleven of the restriction enzymes revealed polymorphism and yielded 16 mitochondrial genotypes or haplotypes. Two of the haplotypes were widely distributed, haplotype 1 being found scattered across southern Mexico and haplotype 2 along the west coast of Mexico. Haplotype 1 also appeared paired with several other haplotypes in mixed lines that were most likely the result of collecting an egg mass to which more than one female had contributed or to some form of contamination by haplotype 1 after introduction into the laboratory. These lines became fixed before single insects were examined and thus it is impossible to rule out heteroplasmy. The other 14 haplotypes were found in only a single locale and 12 of these were found in only one line. The average sequence diversity among 27 mainland lines was about 0.5%. The two Jamaican lines and one east coast mainland line differed from the others by >2%. The pattern of geographical distribution, a small number of apparently recurring haplotypes and a substantial number (75%) of the haplotypes unique, bears similarities to patterns observed in other insects such asDrosophila. The high frequency of unique genotypes in southern Mexico suggests a population with a very reduced gene flow, which may have had a positive effect on the sterile male release control program. Plenum Publishing Corporation, 1989 mitochondrial DNA (mtDNA) nationallicence restriction fragment analysis nationallicence genetic variation nationallicence screwworm fly nationallicence Diptera nationallicence Cochliomyia hominivorax nationallicence Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 27/9-10(1989-10-01), 551-569 0006-2928 27:9-10<551 1989 27 10528 https://doi.org/10.1007/BF00553632 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00553632 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Roehrdanz Richard United States Department of Agriculture, Agricultural Research Service, Biosciences Research Laboratory, State University Station, 58105, Fargo, North Dakota aut NATIONALLICENCE 773 0- Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 27/9-10(1989-10-01), 551-569 0006-2928 27:9-10<551 1989 27 10528 NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02396152 text/html Onlinezugriff via DOI Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer