caa a22 4500 467947120 CHVBK 20180323165701.0 cr unu---uuuuu 170328e19880901xx s 000 0 eng 10.1007/BF00939311 doi (NATIONALLICENCE)springer-10.1007/BF00939311 (NATIONALLICENCE)springer-10.1007/BF01982906 The involvement of an enantioselective transaminase in the metabolism of D-3- and D-4-hydroxyphenylglycine in Pseudomonas putida LW-4 [Elektronische Daten] [W. van den Tweel, J. Smits, R. Ogg, J. de Bont] Summary: Pseudomonas putida LW-4, isolated on D-phenylglycine as sole carbon and energy source, was also able to grow on D-3-and D-4-hydroxyphenylglycine. Both D-3-and D-4-hydroxyphenylglycine were initially converted to the corresponding hydroxyphenylglyoxylates by means of an enantioselective transaminase. Subsequently, the hydroxyphenylglyoxylates were decarboxylated and then oxidized to 3-and 4-hydroxybenzoate, respectively. These latter compounds in turn were oxidized by NADPH-dependent hydroxylases to protocatechuate, which was further oxidized via an intradiol cleavage. Preliminary experiments with cell extracts in which the 4-hydroxyphenylglyoxylate decarboxylase was partially removed by an ammonium sulfate fractionation showed that D-4-hydroxyphenylglyoxycine could be formed from 4-hydroxyphenylglyoxylate by the enantioselective transaminase. Springer-Verlag, 1988 van den Tweel W. Division of Industrial Microbiology, Department of Food Science, Agricultural University, Bomenweg 2, 6703 HD, Wageningen, The Netherlands aut Smits J. Division of Industrial Microbiology, Department of Food Science, Agricultural University, Bomenweg 2, 6703 HD, Wageningen, The Netherlands aut Ogg R. Andeno B.V., Post Office Box 81, 5900 AB, Venlo, The Netherlands aut de Bont J. Division of Industrial Microbiology, Department of Food Science, Agricultural University, Bomenweg 2, 6703 HD, Wageningen, The Netherlands aut Applied Microbiology and Biotechnology Springer-Verlag 29/2-3(1988-09-01), 224-230 0175-7598 29:2-3<224 1988 29 253 https://doi.org/10.1007/BF00939311 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00939311 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- van den Tweel W. Division of Industrial Microbiology, Department of Food Science, Agricultural University, Bomenweg 2, 6703 HD, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Smits J. Division of Industrial Microbiology, Department of Food Science, Agricultural University, Bomenweg 2, 6703 HD, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Ogg R. Andeno B.V., Post Office Box 81, 5900 AB, Venlo, The Netherlands aut NATIONALLICENCE 700 1- de Bont J. Division of Industrial Microbiology, Department of Food Science, Agricultural University, Bomenweg 2, 6703 HD, Wageningen, The Netherlands aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 29/2-3(1988-09-01), 224-230 0175-7598 29:2-3<224 1988 29 253 NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01982906 text/html Onlinezugriff via DOI Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer