caa a22 4500 46794718X CHVBK 20180323165701.0 cr unu---uuuuu 170328e19880901xx s 000 0 eng 10.1007/BF01982914 doi (NATIONALLICENCE)springer-10.1007/BF01982914 (NATIONALLICENCE)springer-10.1007/BF00939319 Isolation of a new cellulase gene from a thermophilic anaerobe and its expression in Escherichia coli [Elektronische Daten] [Hiroyuki Honda, Takao Saito, Shinji Iijima, Takeshi Kobayashi] Summary: A new cellulase gene was cloned and expressed inEscherichia coli from a thermophilic anaerobe, strain NA10. A 7.4 kbEcoRI fragment of NA10 DNA encoded the cellulase which hydrolyzed carboxymethyl cellulose, lichenan, andp-nitrophenyl-β-d-cellobioside, but could not digest laminarin andp-nitrophenyl-β-d-glucoside. The cloned enzyme could digest cellooligosaccharides and release cellobiose as a main product from cellotetraose but could not digest cellobiose. It was distinct from the endoglucanase which was cloned by us previously from NA10 strain in terms ofp-nitrophenyl-β-d-cellobioside degradation activity and the location of restriction enzyme sites. The enzyme produced byE. coli transformant was extremely heat-stable and the optimum temperature for the enzymatic reaction was 80°C. Fifty three percent of the cloned enzyme was detected in the periplasm and the remaining activity existed in the cellular fraction in theE. coli transformant. Springer-Verlag, 1988 Honda Hiroyuki Department of Chemical Engineering, Faculty of Engineering, Nagoya University, Chikusa-ku, 464, Nagoya, Japan aut Saito Takao Department of Chemical Engineering, Faculty of Engineering, Nagoya University, Chikusa-ku, 464, Nagoya, Japan aut Iijima Shinji Department of Chemical Engineering, Faculty of Engineering, Nagoya University, Chikusa-ku, 464, Nagoya, Japan aut Kobayashi Takeshi Department of Chemical Engineering, Faculty of Engineering, Nagoya University, Chikusa-ku, 464, Nagoya, Japan aut Applied Microbiology and Biotechnology Springer-Verlag 29/2-3(1988-09-01), 264-268 0175-7598 29:2-3<264 1988 29 253 https://doi.org/10.1007/BF01982914 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01982914 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Honda Hiroyuki Department of Chemical Engineering, Faculty of Engineering, Nagoya University, Chikusa-ku, 464, Nagoya, Japan aut NATIONALLICENCE 700 1- Saito Takao Department of Chemical Engineering, Faculty of Engineering, Nagoya University, Chikusa-ku, 464, Nagoya, Japan aut NATIONALLICENCE 700 1- Iijima Shinji Department of Chemical Engineering, Faculty of Engineering, Nagoya University, Chikusa-ku, 464, Nagoya, Japan aut NATIONALLICENCE 700 1- Kobayashi Takeshi Department of Chemical Engineering, Faculty of Engineering, Nagoya University, Chikusa-ku, 464, Nagoya, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 29/2-3(1988-09-01), 264-268 0175-7598 29:2-3<264 1988 29 253 NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00939319 text/html Onlinezugriff via DOI Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer