caa a22 4500 46794783X CHVBK 20180405193739.0 cr unu---uuuuu 170328e20041001xx s 000 0 eng 10.1007/s10725-004-4031-z doi (NATIONALLICENCE)springer-10.1007/s10725-004-4031-z (NATIONALLICENCE)springer-10.1023/B:GROW.0000049418.92833.d6 Localization of antioxidant enzymes in the cellular compartments of sorghum leaves [Elektronische Daten] [Durai Sundar, B. Perianayaguy, A. Reddy] The localization of antioxidant enzymes between the mesophyll and bundle sheath cells were determined in sorghum (Sorghum vulgare L.) leaves. The activity of antioxidant enzymes like superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (POD), ascorbate peroxidase (APX) and glutathione reductase (GR) were assayed in whole leaf, mesophyll and bundle sheath fractions of sorghum leaves subjected to water-limited conditions. Drought was imposed by withholding water and the plants were maintained at different water potentials ranging from 0.5-2.0MPa. The purity of the isolates was tested using the marker enzymes like RuBPcase and PEPcase. GR was mostly localized in mesophyll fraction, while SOD, APX and peroxidase were located in bundle sheath cells. Catalase was found to be equally distributed between the two cell types. Under water stress conditions, most of the SOD activity was found in the bundle sheath tissues. Little or no activity of the enzymes CAT, APX or POD was found in the mesophyll extracts when exposed to water stress. GR activity increased when exposed to low water regimes. From this study, it is clear that antioxidants are differentially distributed between the mesophyll and bundle sheath cells in sorghum leaves. Under water stress conditions, the mesophyll cells showed less damage from oxidative stress when compared to the bundle sheath cells. This is critical for determining the sensitivity of sorghum to extreme climatic conditions. Kluwer Academic Publishers, 2004 AOS : active oxygen species nationallicence APX : active oxygen species nationallicence BS : bundle sheath cells nationallicence CAT : catalase nationallicence GR : glutathione reductase nationallicence M : mesophyll cells nationallicence MDA : monodehydroascorbate nationallicence MDHAR : monodehydroascorbate reductase nationallicence PEPcase : phosphenol-pyruvate carboxylase nationallicence POD : peroxidase nationallicence RuBPCase : Ribulose-bisphosphate carboxylase nationallicence SOD : superoxide dismutase nationallicence Antioxidant enzymes nationallicence Bundle sheath nationallicence Mesophyll nationallicence Sorghum vulgare nationallicence Sundar Durai School of Life Sciences, Pondicherry University, 605 014, Pondicherry, India aut Perianayaguy B. School of Life Sciences, Pondicherry University, 605 014, Pondicherry, India aut Reddy A. School of Life Sciences, Pondicherry University, 605 014, Pondicherry, India aut Plant Growth Regulation Kluwer Academic Publishers 44/2(2004-10-01), 157-163 0167-6903 44:2<157 2004 44 10725 https://doi.org/10.1007/s10725-004-4031-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-004-4031-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sundar Durai School of Life Sciences, Pondicherry University, 605 014, Pondicherry, India aut NATIONALLICENCE 700 1- Perianayaguy B. School of Life Sciences, Pondicherry University, 605 014, Pondicherry, India aut NATIONALLICENCE 700 1- Reddy A. School of Life Sciences, Pondicherry University, 605 014, Pondicherry, India aut NATIONALLICENCE 773 0- Plant Growth Regulation Kluwer Academic Publishers 44/2(2004-10-01), 157-163 0167-6903 44:2<157 2004 44 10725 NATIONALLICENCE 700 1- Sundar Durai School of Life Sciences, Pondicherry University, Pondicherry -, 605 014, India (Authors for correspondence (e-mail aut NATIONALLICENCE 700 1- Perianayaguy B. School of Life Sciences, Pondicherry University, Pondicherry -, 605 014, India (Authors for correspondence (e-mail aut NATIONALLICENCE 700 1- Reddy A. School of Life Sciences, Pondicherry University, Pondicherry -, 605 014, India (Authors for correspondence (e-mail aut NATIONALLICENCE 856 40 https://doi.org/10.1023/B:GROW.0000049418.92833.d6 text/html Onlinezugriff via DOI Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer