caa a22 4500 467947929 CHVBK 20180323165701.0 cr unu---uuuuu 170328e19881001xx s 000 0 eng 10.1007/PL00020496 doi (NATIONALLICENCE)springer-10.1007/PL00020496 (NATIONALLICENCE)springer-10.1007/BF02399601 Mapping of mouse gamma crystallin genes on chromosome 1 [Elektronische Daten] [Loren Skow, Maria Donner, Shu-Mei Huang, John Gardner, Benjamin Taylor, Wesley Beamer, Peter Lalley] Restriction fragments analysis of DNA from mouse-hamster somatic-cell hybrid clones revealed that a mouse gamma crystallin cDNA hybridized to genomic sequences located on mouse chromosome 1. Identification of restriction fragment length polymorphisms (RFLPs) in the gamma crystallin sequences of inbred strains of mice permitted the further localization of the gamma crystallin genes (Cryg) to the proximal region of chromosome 1 closely linked to the loci encoding isocitrate dehydrogenase (Idh-1), a low molecular weight (LM) crystallin protein polymorphism (Len-1), and fibronectin (Fn-1). A single recombinant was observed betweenLen-1 and an RFLP in the gamma crystallin gene family, consistent with the hypothesis thatLen-1 is one of the several structural loci encoding gamma crystallin genes.Len-1 is probably located on the centromeric end of theCryg gene family. Linkage ofIdh-1, Cryg, andFn-1 in mice extends the syntenic relationship of those loci to the human, bovine, and rodent genomes and may define a chromosomal region that is generally conserved among mammals. The map position ofCryg, near the eye lens obsolescence (Elo) locus, was confirmed by the discovery that the restriction fragment patterns of gamma crystallin sequences differed between strain C3H/HeJ and the congenic anophthalmic mutant strain, C3H.Elo. Therefore, the gamma crystallin genes were contransferred with the mutantElo gene in the derivation of C3H.Elo. The results establish that LEN-1 is a marker for the gamma crystallin gene family, position the gamma crystallin gene family relative to other markers on mouse chromosome 1, and provide additional evidence that theElo mutation is encoded at a locus closely linked to the gamma crystallin gene cluster. This study found no evidence of recombination hot spots within the gamma crystallin gene cluster. Plenum Publishing Corporation, 1988 mouse nationallicence gamma crystallin nationallicence gene mapping nationallicence Skow Loren Department of Veterinary Anatomy, College of Veterinary Medicine, Texas A&M University, 77843-4458, College Station, Texas aut Donner Maria Laboratory of Geneties, National Institute of Environmental Health Sciences, 27709, Research Triangle Park, North Carolina aut Huang Shu-Mei Laboratory of Geneties, National Institute of Environmental Health Sciences, 27709, Research Triangle Park, North Carolina aut Gardner John The Jackson Laboratory, 04605, Bar Harbor, Maine aut Taylor Benjamin The Jackson Laboratory, 04605, Bar Harbor, Maine aut Beamer Wesley The Jackson Laboratory, 04605, Bar Harbor, Maine aut Lalley Peter Institute for Medical Research, 05201, Bennington, Vermont aut Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 26/9-10(1988-10-01), 557-570 0006-2928 26:9-10<557 1988 26 10528 https://doi.org/10.1007/PL00020496 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00020496 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Skow Loren Department of Veterinary Anatomy, College of Veterinary Medicine, Texas A&M University, 77843-4458, College Station, Texas aut NATIONALLICENCE 700 1- Donner Maria Laboratory of Geneties, National Institute of Environmental Health Sciences, 27709, Research Triangle Park, North Carolina aut NATIONALLICENCE 700 1- Huang Shu-Mei Laboratory of Geneties, National Institute of Environmental Health Sciences, 27709, Research Triangle Park, North Carolina aut NATIONALLICENCE 700 1- Gardner John The Jackson Laboratory, 04605, Bar Harbor, Maine aut NATIONALLICENCE 700 1- Taylor Benjamin The Jackson Laboratory, 04605, Bar Harbor, Maine aut NATIONALLICENCE 700 1- Beamer Wesley The Jackson Laboratory, 04605, Bar Harbor, Maine aut NATIONALLICENCE 700 1- Lalley Peter Institute for Medical Research, 05201, Bennington, Vermont aut NATIONALLICENCE 773 0- Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 26/9-10(1988-10-01), 557-570 0006-2928 26:9-10<557 1988 26 10528 NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02399601 text/html Onlinezugriff via DOI Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer