caa a22 4500 467948224 CHVBK 20180323165701.0 cr unu---uuuuu 170328e19881201xx s 000 0 eng 10.1007/PL00020550 doi (NATIONALLICENCE)springer-10.1007/PL00020550 (NATIONALLICENCE)springer-10.1007/BF02395514 Method for quantitating cholesterol in subfractions of serum lipoproteins separated by gradient gel electrophoresis [Elektronische Daten] [M. Cheng, C. Kammerer, W. Lowe, B. Dyke, J. VandeBerg] Extensive heterogeneity in particle size distribution of serum lipoproteins of baboons was resolved by a procedure that combined Sudan black B prestaining, polyacrylamide gradient gel electrophoresis (GGE), and quantitative densitometry. Each densitometric scan represented a continuous distribution of the relative amount of cholesterol in a serum sample, as a function of the lipoprotein particle size. For analytical purposes, each scan was divided into 12 fractions, representing 12 particle size ranges. The relationship between the estimated cholesterol concentrations in the summed GGE/densitometric fractions corresponding to very low-density lipoproteins (VLDL) + low-density lipoproteins (LDL) and those corresponding to high-density lipoproteins (HDL) and concentrations measured by the heparin-Mn2+ precipitation/enzymatic procedure was linear over a broad range. However, a systematic overestimation of HDL cholesterol concentration and an underestimation of VLDL + LDL cholesterol concentration was apparent. Therefore, correction factors were developed for adjusting the estimates of VLDL + LDL and HDL cholesterol concentrations obtained by the GGE/densitometric method. This analytical method is rapid, repeatable, economical, and useful for genetic and dietary research in which cholesterol concentrations in multiple particle size ranges of lipoproteins must be measured in large numbers of samples. It also is adaptable to immunoblotting procedures for detecting the distribution of specific apolipoproteins among the size-resolved lipoproteins. Plenum Publishing Corporation, 1988 gradient gel electrophoresis nationallicence quantitative densitometry nationallicence serum lipoprotein cholesterol nationallicence primates nationallicence Cheng M. Department of Genetics, Southwest Foundation for Biomedical Research, P.O. Box 28147, 78284-2500, San Antonio, Texas aut Kammerer C. Department of Genetics, Southwest Foundation for Biomedical Research, P.O. Box 28147, 78284-2500, San Antonio, Texas aut Lowe W. Department of Genetics, Southwest Foundation for Biomedical Research, P.O. Box 28147, 78284-2500, San Antonio, Texas aut Dyke B. Department of Genetics, Southwest Foundation for Biomedical Research, P.O. Box 28147, 78284-2500, San Antonio, Texas aut VandeBerg J. Department of Genetics, Southwest Foundation for Biomedical Research, P.O. Box 28147, 78284-2500, San Antonio, Texas aut Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 26/11-12(1988-12-01), 657-681 0006-2928 26:11-12<657 1988 26 10528 https://doi.org/10.1007/PL00020550 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00020550 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cheng M. Department of Genetics, Southwest Foundation for Biomedical Research, P.O. Box 28147, 78284-2500, San Antonio, Texas aut NATIONALLICENCE 700 1- Kammerer C. Department of Genetics, Southwest Foundation for Biomedical Research, P.O. Box 28147, 78284-2500, San Antonio, Texas aut NATIONALLICENCE 700 1- Lowe W. Department of Genetics, Southwest Foundation for Biomedical Research, P.O. Box 28147, 78284-2500, San Antonio, Texas aut NATIONALLICENCE 700 1- Dyke B. Department of Genetics, Southwest Foundation for Biomedical Research, P.O. Box 28147, 78284-2500, San Antonio, Texas aut NATIONALLICENCE 700 1- VandeBerg J. Department of Genetics, Southwest Foundation for Biomedical Research, P.O. Box 28147, 78284-2500, San Antonio, Texas aut NATIONALLICENCE 773 0- Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 26/11-12(1988-12-01), 657-681 0006-2928 26:11-12<657 1988 26 10528 NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02395514 text/html Onlinezugriff via DOI Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer