caa a22 4500 467949158 CHVBK 20180323165701.0 cr unu---uuuuu 170328e19891001xx s 000 0 eng 10.1007/BF02396154 doi (NATIONALLICENCE)springer-10.1007/BF02396154 (NATIONALLICENCE)springer-10.1007/BF00553634 Novel restriction fragment length polymorphism of the growth hormone gene in inbred rats [Elektronische Daten] [Tetsuo Kunieda, Hiroshi Ikadai, Minami Matsui, Nobuo Nomura, Tomonori Imamichi, Ryotaro Ishizaki] A novel restriction fragment length polymorphism in inbred rats was detected by Southern blot analysis with rat growth hormone cDNA as a probe. Four alleles, characterized byPstI fragments of 1.2, 1.1, 0.9, and 0.7 kb, respectively, were detected in 27 strains examined. The same distribution of polymorphisms was observed on digestion of DNAs of these strains with three other enzymes,PvuII,HindIII, andBamHI. Moreover, the same differences in length of allelic restriction fragments were obtained with these restriction enzymes as withPstI. These findings suggested that the polymorphism was caused by insertion or deletion of variable DNA segments in the second intron of the growth hormone gene. Linkage analyses using backcross progeny provided no evidence for close linkage between the restriction fragment length polymorphism locus and 10 other loci examined. Plenum Publishing Corporation, 1989 mammalian genetics nationallicence Rattus norvegicus nationallicence growth hormone gene nationallicence restriction fragment length polymorphism nationallicence Kunieda Tetsuo Imamichi Institute for Animal Reproduction, 100, Nisshin-cho 3-chome, Ohmiya-shi, 330, Saitama, Japan aut Ikadai Hiroshi Imamichi Institute for Animal Reproduction, 100, Nisshin-cho 3-chome, Ohmiya-shi, 330, Saitama, Japan aut Matsui Minami Molecular Oncology Laboratory, Nippon Veterinary and Zootechnical College, 10-19, Uenosakuragi 1-chome, Taito-ku, 110, Tokyo, Japan aut Nomura Nobuo Molecular Oncology Laboratory, Nippon Veterinary and Zootechnical College, 10-19, Uenosakuragi 1-chome, Taito-ku, 110, Tokyo, Japan aut Imamichi Tomonori Imamichi Institute for Animal Reproduction, 100, Nisshin-cho 3-chome, Ohmiya-shi, 330, Saitama, Japan aut Ishizaki Ryotaro Molecular Oncology Laboratory, Nippon Veterinary and Zootechnical College, 10-19, Uenosakuragi 1-chome, Taito-ku, 110, Tokyo, Japan aut Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 27/9-10(1989-10-01), 591-602 0006-2928 27:9-10<591 1989 27 10528 https://doi.org/10.1007/BF02396154 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02396154 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kunieda Tetsuo Imamichi Institute for Animal Reproduction, 100, Nisshin-cho 3-chome, Ohmiya-shi, 330, Saitama, Japan aut NATIONALLICENCE 700 1- Ikadai Hiroshi Imamichi Institute for Animal Reproduction, 100, Nisshin-cho 3-chome, Ohmiya-shi, 330, Saitama, Japan aut NATIONALLICENCE 700 1- Matsui Minami Molecular Oncology Laboratory, Nippon Veterinary and Zootechnical College, 10-19, Uenosakuragi 1-chome, Taito-ku, 110, Tokyo, Japan aut NATIONALLICENCE 700 1- Nomura Nobuo Molecular Oncology Laboratory, Nippon Veterinary and Zootechnical College, 10-19, Uenosakuragi 1-chome, Taito-ku, 110, Tokyo, Japan aut NATIONALLICENCE 700 1- Imamichi Tomonori Imamichi Institute for Animal Reproduction, 100, Nisshin-cho 3-chome, Ohmiya-shi, 330, Saitama, Japan aut NATIONALLICENCE 700 1- Ishizaki Ryotaro Molecular Oncology Laboratory, Nippon Veterinary and Zootechnical College, 10-19, Uenosakuragi 1-chome, Taito-ku, 110, Tokyo, Japan aut NATIONALLICENCE 773 0- Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 27/9-10(1989-10-01), 591-602 0006-2928 27:9-10<591 1989 27 10528 NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00553634 text/html Onlinezugriff via DOI Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer