caa a22 4500 467949220 CHVBK 20180323165701.0 cr unu---uuuuu 170328e19891201xx s 000 0 eng 10.1007/BF02396058 doi (NATIONALLICENCE)springer-10.1007/BF02396058 (NATIONALLICENCE)springer-10.1007/BF00553987 Nucleotide ectoenzyme activities of human and Chinese hamster fibroblasts in tissue culture [Elektronische Daten] [J. Boyle, Y. Hey, M. Fox] We have previously assigned human ecto-5′-nucleotidase (NT) to chromosome 6 on the basis of conversion of exogenously supplied [14C] AMP to adenosine by whole cells of human and Chinese hamster hybrids carrying chromosome 6. In this paper we demonstrate that the activity on human MRC-5 fibroblasts is typical of previously described and purified ecto-5′-nucleotidases. In contrast to MRC-5 cells, Chinese hamster V79A2 cells weakly express an AMPase activity that is not NT. The cytosolic form of NT in human and hybrid fibroblasts is similar to the ectoenzyme in substrate specificity. Hybrids that lack chromosome 6 express neither the ecto- nor the cytosolic enzyme, suggesting that both forms may be coded by the same gene on chromosome 6. Ecto-ATPase, ecto-ADPase, and ecto-ADP kinase activities are each expressed at similar levels in MRC-5 and V79A2. The ATPase, ADPase and NT activities of MRC-5 cells act sequentially to generate adenosine. A similar cascade acts on V79A2 cells but the lack of NT causes the accumulation of AMP. Plenum Publishing Corporation, 1989 5′-nucleotidase nationallicence biochemical genetics nationallicence purine salvage nationallicence ectoenzyme nationallicence Boyle J. Department of Biochemical Genetics, Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, M20 9BX, Manchester, U.K. aut Hey Y. Department of Biochemical Genetics, Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, M20 9BX, Manchester, U.K. aut Fox M. Department of Biochemical Genetics, Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, M20 9BX, Manchester, U.K. aut Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 27/11-12(1989-12-01), 655-671 0006-2928 27:11-12<655 1989 27 10528 https://doi.org/10.1007/BF02396058 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02396058 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Boyle J. Department of Biochemical Genetics, Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, M20 9BX, Manchester, U.K aut NATIONALLICENCE 700 1- Hey Y. Department of Biochemical Genetics, Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, M20 9BX, Manchester, U.K aut NATIONALLICENCE 700 1- Fox M. Department of Biochemical Genetics, Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, M20 9BX, Manchester, U.K aut NATIONALLICENCE 773 0- Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 27/11-12(1989-12-01), 655-671 0006-2928 27:11-12<655 1989 27 10528 NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00553987 text/html Onlinezugriff via DOI Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer