caa a22 4500 467949522 CHVBK 20180323165702.0 cr unu---uuuuu 170328e19880901xx s 000 0 eng 10.1007/BF01982904 doi (NATIONALLICENCE)springer-10.1007/BF01982904 (NATIONALLICENCE)springer-10.1007/BF00939309 Optimization of lipid production in the oleaginous yeast Apiotrichum curvatum in wheypermeate [Elektronische Daten] [Adrie Ykema, Elizabeth Verbree, Martin Kater, Henk Smit] Summary: Lipid production of the oleaginous yeastApiotrichum curvatum was studied in wheypermeate to determine optimum operation conditions in this medium. Studies on the influence of the carbon to nitrogen ratio (C/N-ratio) of the growth medium on lipid production in continuous cultures demonstrated that cellular lipid content in wheypermeate remained constant at 22% of the cell dry weight up to a C/N-ratio of about 25. The maximal dilution rate at which all lactose is consumed in wheypermeate with excess nitrogen was found to be 0.073 h-1. At C/N-ratios higher than 25-30 lipid content gradually increased to nearly 50% at C/N=70 and the maximal obtainable dilution rate decreased to 0.02 h-1 at C/N=70. From these studies it could be derived that maximal lipid production rates can be obtained at C/N-ratios of 30-35 in wheypermeate. Since the C/N-ratio of wheypermeate normally has a value between 70 and 101, some additional nitrogen is required to optimize the lipid production rate. Lipid production rates ofA. curvatum in wheypermeate were compared in four different culture modes: batch, fed-batch, continuous and partial recycling cultures. Highest lipid production rates were achieved in culture modes with high cell densities. A lipid production rate of nearly 1 g/l/h was reached in a partial recycling culture. It was calculated that by using this cultivation technique lipid production rates of even 2.9 g/l/h may be reached when the supply of oxygen can be optimized. Springer-Verlag, 1988 Ykema Adrie Department of Genetics, Biological Laboratory, Vrije Universiteit, de Boelelaan 1087, 1081 HV, Amsterdam, The Netherlands aut Verbree Elizabeth Department of Genetics, Biological Laboratory, Vrije Universiteit, de Boelelaan 1087, 1081 HV, Amsterdam, The Netherlands aut Kater Martin Department of Genetics, Biological Laboratory, Vrije Universiteit, de Boelelaan 1087, 1081 HV, Amsterdam, The Netherlands aut Smit Henk Department of Genetics, Biological Laboratory, Vrije Universiteit, de Boelelaan 1087, 1081 HV, Amsterdam, The Netherlands aut Applied Microbiology and Biotechnology Springer-Verlag 29/2-3(1988-09-01), 211-218 0175-7598 29:2-3<211 1988 29 253 https://doi.org/10.1007/BF01982904 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01982904 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ykema Adrie Department of Genetics, Biological Laboratory, Vrije Universiteit, de Boelelaan 1087, 1081 HV, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Verbree Elizabeth Department of Genetics, Biological Laboratory, Vrije Universiteit, de Boelelaan 1087, 1081 HV, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Kater Martin Department of Genetics, Biological Laboratory, Vrije Universiteit, de Boelelaan 1087, 1081 HV, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Smit Henk Department of Genetics, Biological Laboratory, Vrije Universiteit, de Boelelaan 1087, 1081 HV, Amsterdam, The Netherlands aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 29/2-3(1988-09-01), 211-218 0175-7598 29:2-3<211 1988 29 253 NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00939309 text/html Onlinezugriff via DOI Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer