caa a22 4500 467950334 CHVBK 20180323165702.0 cr unu---uuuuu 170328e19880901xx s 000 0 eng 10.1007/BF00939314 doi (NATIONALLICENCE)springer-10.1007/BF00939314 (NATIONALLICENCE)springer-10.1007/BF01982909 Production of acid proteinases by Humicola lutea immobilized in polyacrylamide gel [Elektronische Daten] [P. Aleksieva, B. Tchorbanov, G. Suchodol'skaya, K. Koschteenko] Summary: Fungal spores ofHumicola lutea 120-5 were entrapped in 5% polyacrylamide gel and were cultivated for 44-48 h to form a mycelial network inside the beads. A dense mycelial growth also occurred on the surface of the beads. It was possible to reuse the immobilized mycelium for production of acid proteinases in 12 different batches without loss of mechanical stability. The inoculum size should be controlled prior to its transfer into fresh production medium. Maximal enzyme production exceeding the level of free cell fermentation was registered in the fourth to seventh cycles. According to the size of the inoculum, half of the initial production rate was reached after 7-14 batches. Springer-Verlag, 1988 Aleksieva P. Institute of Microbiology, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria aut Tchorbanov B. Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria aut Suchodol'skaya G. Institute of Physiology and Biochemistry of Microorganisms, Academy of Sciences of the USSR, 142292, Pushchino na Oke, USSR aut Koschteenko K. Institute of Physiology and Biochemistry of Microorganisms, Academy of Sciences of the USSR, 142292, Pushchino na Oke, USSR aut Applied Microbiology and Biotechnology Springer-Verlag 29/2-3(1988-09-01), 239-241 0175-7598 29:2-3<239 1988 29 253 https://doi.org/10.1007/BF00939314 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00939314 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Aleksieva P. Institute of Microbiology, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria aut NATIONALLICENCE 700 1- Tchorbanov B. Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria aut NATIONALLICENCE 700 1- Suchodol'skaya G. Institute of Physiology and Biochemistry of Microorganisms, Academy of Sciences of the USSR, 142292, Pushchino na Oke, USSR aut NATIONALLICENCE 700 1- Koschteenko K. Institute of Physiology and Biochemistry of Microorganisms, Academy of Sciences of the USSR, 142292, Pushchino na Oke, USSR aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 29/2-3(1988-09-01), 239-241 0175-7598 29:2-3<239 1988 29 253 NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01982909 text/html Onlinezugriff via DOI Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer