caa a22 4500 467950512 CHVBK 20180323165702.0 cr unu---uuuuu 170328e19881001xx s 000 0 eng 10.1007/PL00020495 doi (NATIONALLICENCE)springer-10.1007/PL00020495 (NATIONALLICENCE)springer-10.1007/BF02399600 Purification and characterization of cytoplasmic creatine kinase isozymes of Xenopus laevis [Elektronische Daten] [J. Robert, H. Kobel] The soluble creatine kinase isozymes CK-II, CK-III, and CK-IV fromXenopus laevis have been purified to apparent homogeneity and their subunits characterized by means of molecular weight, peptide pattern, and dissociation-reassociation experiments. CK-III and CK-IV are homodimeric isozymes whose subunits are distinct in both molecular weight (42,000 and 41,000, respectively) andStaphylococcus aureus V8 peptide pattern. In dissociation-reassociation experiments, those two subunits do form active heterodimeric isozymes with one another or with rabbit M-CK subunits. Hybrid CK-III/IV isozymes occur also during embryonic differentiation and in adult heart muscle, whereas most other adult tissues contain only homodimeric CK-III or CK-IV isozymes. The CK-II isozyme is a heterodimer composed of one CK-III subunit and another subunit specific to CK-II (M r =41,000). Neitherin vivo norin vitro does this subunit seem able to form homodimers or heterodimers with CK-IV and rabbit M-CK subunits. If we take into account the apparent association of CK-I isozyme with cellular organelles, these results corroborate earlier statements and suggest that the CK isozyme system ofX. laevis is encoded by at least four differentially regulated genomic loci. Plenum Publishing Corporation, 1988 creatinine kinase nationallicence Xenopus laevis nationallicence fingerprinting nationallicence molecular weight nationallicence reversible dissociation nationallicence Robert J. Laboratoire de Génétique Animale et Végétale, Université de Genève, CH-1224, Chêne-Bougeries, Switzerland aut Kobel H. Laboratoire de Génétique Animale et Végétale, Université de Genève, CH-1224, Chêne-Bougeries, Switzerland aut Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 26/9-10(1988-10-01), 543-555 0006-2928 26:9-10<543 1988 26 10528 https://doi.org/10.1007/PL00020495 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00020495 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Robert J. Laboratoire de Génétique Animale et Végétale, Université de Genève, CH-1224, Chêne-Bougeries, Switzerland aut NATIONALLICENCE 700 1- Kobel H. Laboratoire de Génétique Animale et Végétale, Université de Genève, CH-1224, Chêne-Bougeries, Switzerland aut NATIONALLICENCE 773 0- Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 26/9-10(1988-10-01), 543-555 0006-2928 26:9-10<543 1988 26 10528 NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02399600 text/html Onlinezugriff via DOI Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer