caa a22 4500 467950628 CHVBK 20180405193741.0 cr unu---uuuuu 170328e20040101xx s 000 0 eng 10.1007/s00280-003-0702-0 doi (NATIONALLICENCE)springer-10.1007/s00280-003-0702-0 (NATIONALLICENCE)springer-10.1007/BF02665357 Quercetin inhibits the invasion of murine melanoma B16-BL6 cells by decreasing pro-MMP-9 via the PKC pathway [Elektronische Daten] [Xian-Ming Zhang, Shao-Peng Huang, Qiang Xu] Purpose: On the basis of the inhibitory effect of quercetin on the invasion of melanoma B16-BL6 cells previously reported by us, the mechanisms of quercetin-mediated inhibition of invasion were further investigated in the present study. Methods: The ability of B16-BL6 cells to invade and migrate was evaluated in terms of the numbers of cells penetrating a reconstituted basement membrane in the Transwell coculture system. The relative levels and activities of matrix metalloproteinase-9 (MMP-9) and MMP-2 were determined by gelatin zymography and quantified using LabWorks 4.0 software. Results: The quercetin-mediated inhibition of invasion was partially blocked by phorbol-12,13-dibutyrate (PDB), a PKC (protein kinase C) activator, and by doxorubicin, a PKC inhibitor. Only the proforms of MMP-9 (92kDa) and MMP-2 (72kDa) were detected by gelatin zymography. Quercetin dose-dependently decreased the gelatinolytic activity of pro-MMP-9. Doxorubicin also markedly reversed the quercetin-induced decrease. Quercetin showed a dose-dependent antagonism of increases in gelatinolytic activity of pro-MMP-9 induced by PDB and free fatty acid (another PKC activator). Conclusions: Together with the report that quercetin directly reduces PKC activity, the results reported here suggest that quercetin may inhibit the invasion of B16-BL6 cells by decreasing pro-MMP-9 via the PKC pathway. Springer-Verlag, 2003 Quercetin nationallicence Melanoma B16-BL6 cells nationallicence MMP nationallicence PKC nationallicence Invasion nationallicence Zhang Xian-Ming State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, The People's Republic of China aut Huang Shao-Peng State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, The People's Republic of China aut Xu Qiang State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, The People's Republic of China aut Cancer Chemotherapy and Pharmacology Springer-Verlag 53/1(2004-01-01), 82-88 0344-5704 53:1<82 2004 53 280 https://doi.org/10.1007/s00280-003-0702-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00280-003-0702-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Xian-Ming State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, The People's Republic of China aut NATIONALLICENCE 700 1- Huang Shao-Peng State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, The People's Republic of China aut NATIONALLICENCE 700 1- Xu Qiang State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, The People's Republic of China aut NATIONALLICENCE 773 0- Cancer Chemotherapy and Pharmacology Springer-Verlag 53/1(2004-01-01), 82-88 0344-5704 53:1<82 2004 53 280 NATIONALLICENCE 700 1- Zhang Xian-Ming State Key Laboratory of Pharmaceutical Biotechnology school of Life Sciences, Nanjing University, 210093, Nanjing, The People's Republic of China aut NATIONALLICENCE 700 1- Huang Shao-Peng State Key Laboratory of Pharmaceutical Biotechnology school of Life Sciences, Nanjing University, 210093, Nanjing, The People's Republic of China aut NATIONALLICENCE 700 1- Xu Qiang State Key Laboratory of Pharmaceutical Biotechnology school of Life Sciences, Nanjing University, 210093, Nanjing, The People's Republic of China aut NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02665357 text/html Onlinezugriff via DOI Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer