caa a22 4500 467950997 CHVBK 20180323165702.0 cr unu---uuuuu 170328e19891201xx s 000 0 eng 10.1007/BF00553990 doi (NATIONALLICENCE)springer-10.1007/BF00553990 (NATIONALLICENCE)springer-10.1007/BF02396061 Restriction fragment length variations and chromosome mapping of two mouse metallothionein genes, Mt-1 and Mt-2 [Elektronische Daten] [Tomomasa Watanabe, Atsuko Shimizu, Kyoko Ohno, Shigeo Masaki, Kyoji Kondo] Restriction endonuclease fragment length variations (RFLVs) were found through the use of cDNA probes for metallothionein genes 1 (Mt-1) and 2 (Mt-2) in the mouse. RFLVs were detected in restriction patterns generated byBglII andXbaI in theMt-1 gene and byPvuII in theMt-2 gene. All laboratory strains carry theMt-1 a andMt-2 a alleles. Among strains of wild origin, some Western European subspecies (Mus mus domesticus andM. m. brevirostris) also carry theMt-1 a andMt-2 a alleles. In contrast, a European subspecies (M. m. musculus) and the great majority of subspecies from East Asian countries (M. m. molossinus, Chinese mice of wild origin, andM. m. yamashinai) carry theMt-1 b andMt-2 b alleles. Adomesticus strain from Bulgaria and twocastaneus strains from Thailand and Philippines carry the intermediate combination ofMt-1 b andMt-2 a alleles. Using the RFLVs, we mapped theMt-1 andMt-2 genes on chromosome 8, and they appear to be very closely linked since no recombination was observed between them in any of the mice examined. Data from three-point cross tests showed that the recombination frequencies are 4.31% betweenOs andMt, 15.52% betweenMt andPrt-2, and 19.83% betweenOs andPrt-2. The gene order ofOs-Mt-1,Mt-2-Prt-2 has been confirmed. Plenum Publishing Corporation, 1989 restriction fragment length variation nationallicence metallothionein nationallicence chromosome mapping nationallicence Watanabe Tomomasa Department of Biochemistry, Institute for Developmental Research, Aichi Prefecture Colony, Kamiyacho, 480-03, Kasugai, Aichi, Japan aut Shimizu Atsuko Department of Biochemistry, Institute for Developmental Research, Aichi Prefecture Colony, Kamiyacho, 480-03, Kasugai, Aichi, Japan aut Ohno Kyoko Laboratory of Experimental Animals, Yagi Memorial Park, Mitake, 505-01, Gifu, Japan aut Masaki Shigeo Department of Biochemistry, Institute for Developmental Research, Aichi Prefecture Colony, Kamiyacho, 480-03, Kasugai, Aichi, Japan aut Kondo Kyoji Laboratory of Experimental Animals, Yagi Memorial Park, Mitake, 505-01, Gifu, Japan aut Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 27/11-12(1989-12-01), 689-697 0006-2928 27:11-12<689 1989 27 10528 https://doi.org/10.1007/BF00553990 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00553990 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Watanabe Tomomasa Department of Biochemistry, Institute for Developmental Research, Aichi Prefecture Colony, Kamiyacho, 480-03, Kasugai, Aichi, Japan aut NATIONALLICENCE 700 1- Shimizu Atsuko Department of Biochemistry, Institute for Developmental Research, Aichi Prefecture Colony, Kamiyacho, 480-03, Kasugai, Aichi, Japan aut NATIONALLICENCE 700 1- Ohno Kyoko Laboratory of Experimental Animals, Yagi Memorial Park, Mitake, 505-01, Gifu, Japan aut NATIONALLICENCE 700 1- Masaki Shigeo Department of Biochemistry, Institute for Developmental Research, Aichi Prefecture Colony, Kamiyacho, 480-03, Kasugai, Aichi, Japan aut NATIONALLICENCE 700 1- Kondo Kyoji Laboratory of Experimental Animals, Yagi Memorial Park, Mitake, 505-01, Gifu, Japan aut NATIONALLICENCE 773 0- Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 27/11-12(1989-12-01), 689-697 0006-2928 27:11-12<689 1989 27 10528 NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02396061 text/html Onlinezugriff via DOI Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer