caa a22 4500 467951306 CHVBK 20180323165702.0 cr unu---uuuuu 170328e19881001xx s 000 0 eng 10.1007/BF02399608 doi (NATIONALLICENCE)springer-10.1007/BF02399608 (NATIONALLICENCE)springer-10.1007/PL00020503 Spicer Greg Division of Biological and Medical Research, Argonne National Laboratory, 60439, Argonne, Illinois aut The separation of whale myoglobins with two-dimensional electrophoresis [Elektronische Daten] [Greg Spicer] Five myoglobins (sperm whale, Sei whale, Hubbs' beaked whale, pilot whale, and Amazon River dolphin) were examined using two-dimensional electrophoresis. Previous reports indicated that none of these proteins could be separated by using denaturing (in the presence of 8-9 M urea) isoelectric focusing. This result is confirmed in the present study. However, all the proteins could be separated by using denaturing nonequilibriumpH-gradient electrophoresis in the first dimension. Additionally, all the myoglobins have characteristic mobilities in the second dimension (sodium dodecyl sulfate), but these mobilities do not correspond to the molecular weights of the proteins. We conclude that two-dimensional electrophoresis can be more sensitive to differences in primary protein structure than previous studies indicate and that the assessment seems to be incorrect that this technique can separate only proteins that have a unit charge difference. Plenum Publishing Corporation, 1988 two-dimensional electrophoresis nationallicence isoelectric focusing nationallicence nonequilibrium p H-gradient electrophoresis nationallicence sodium dodecyl sulfate gel electrophoresis nationallicence genetic variation nationallicence myoglobin nationallicence Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 26/9-10(1988-10-01), 645-655 0006-2928 26:9-10<645 1988 26 10528 https://doi.org/10.1007/BF02399608 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02399608 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Spicer Greg Division of Biological and Medical Research, Argonne National Laboratory, 60439, Argonne, Illinois aut NATIONALLICENCE 773 0- Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 26/9-10(1988-10-01), 645-655 0006-2928 26:9-10<645 1988 26 10528 NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00020503 text/html Onlinezugriff via DOI Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer