caa a22 4500 467951551 CHVBK 20180405193742.0 cr unu---uuuuu 170328e20050101xx s 000 0 eng 10.1007/s10541-005-0051-1 doi (NATIONALLICENCE)springer-10.1007/s10541-005-0051-1 (NATIONALLICENCE)springer-10.1007/PL00021756 Spatial coordination of chloroplast and plasma membrane activities in chara cells and its disruption through inactivation of 14-3-3 proteins [Elektronische Daten] [A. Bulychev, P. van den Wijngaard, A. de Boer] In Chara corallina cells exposed to continuous light, external pH (pHo) and photosystem II (PSII) photochemical yield show correlated banding patterns. Photosynthetic activity is low in cell regions producing alkaline zones and high in the acid regions. We addressed the question whether (and how) photosynthetic activity and plasma membrane (PM) H+-pumping and H+-conductance are coupled in the different bands. First, PM H+-pump activity was stimulated with fusicoccin. This resulted in a more acidic pH in the acid bands without disturbing the correlation of photosynthetic electron transport and H+ fluxes across the PM. Next, H+-pump activity was reduced through microinjection of a phosphorylated peptide matching the canonical 14-3-3 binding motif RSTpSTP in the acid cell region. Microinjection induced a rapid (~5 min) rise in pHo by ca. 1.0 unit near the injection site, whereas the injection of the non-phosphorylated peptide had no effect. This pH rise confirms the supposed inhibition of the H+-pump upon the detachment of 14-3-3 proteins from the H+-ATPase. However, the PSII yield in the cell regions corresponding to the new alkaline peak remained high, which violated the normal inverse relations between the pHo and PSII photochemical yield. We conclude that the injection of the competitive inhibitor of the H+ATPase disrupts the balanced operation of PM H+-transport and photosynthetic electron flow and promotes electron flow through alternative pathways. MAIK "Nauka/Interperiodica”, 2005 Chara corallina nationallicence plasma membrane H+-pump nationallicence chlorophyll fluorescence nationallicence photosynthetic electron transport nationallicence 14-3-3 proteins nationallicence fusicoccin nationallicence NR : nitrate reductase nationallicence NR-C : non-phosphorylated peptide matching the 14-3-3 interaction domain in barley nitrate reductase nationallicence NR-P : a similar peptide containing phosphorylated serine nationallicence pHo : external pH near the cell surface nationallicence PM : plasma membrane nationallicence PSII : photosystem II nationallicence ΔF/Fm' : effective quantum yield of PS II photochemistry nationallicence Bulychev A. Department of Biophysics, Faculty of Biology, Lomonosov Moscow State University, 119992, Moscow, Russia aut van den Wijngaard P. Department of Developmental Genetics, Vrije Universiteit, Faculty of Earth and Life Sciences, De Boelelaan 1085, 1081, HV Amsterdam, The Netherlands aut de Boer A. Department of Developmental Genetics, Vrije Universiteit, Faculty of Earth and Life Sciences, De Boelelaan 1085, 1081, HV Amsterdam, The Netherlands aut Biochemistry (Moscow) Nauka/Interperiodica 70/1(2005-01-01), 55-61 0006-2979 70:1<55 2005 70 10541 https://doi.org/10.1007/s10541-005-0051-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10541-005-0051-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bulychev A. Department of Biophysics, Faculty of Biology, Lomonosov Moscow State University, 119992, Moscow, Russia aut NATIONALLICENCE 700 1- van den Wijngaard P. Department of Developmental Genetics, Vrije Universiteit, Faculty of Earth and Life Sciences, De Boelelaan 1085, 1081, HV Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- de Boer A. Department of Developmental Genetics, Vrije Universiteit, Faculty of Earth and Life Sciences, De Boelelaan 1085, 1081, HV Amsterdam, The Netherlands aut NATIONALLICENCE 773 0- Biochemistry (Moscow) Nauka/Interperiodica 70/1(2005-01-01), 55-61 0006-2979 70:1<55 2005 70 10541 NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00021756 text/html Onlinezugriff via DOI Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer