caa a22 4500 467953643 CHVBK 20180323165703.0 cr unu---uuuuu 170328e19891201xx s 000 0 eng 10.1007/BF02396064 doi (NATIONALLICENCE)springer-10.1007/BF02396064 (NATIONALLICENCE)springer-10.1007/BF00553993 Biochemical and genetic studies on GP43, a 43-kD glycoprotein detected immunologically in human urine and serum [Elektronische Daten] [Keiko Mizuta, Toshihiro Yasuda, Koichiro Kishi] A new and previously undescribed glycoprotein with a molecular weight of 43,000 has been isolated from human urine. This protein, designated GP43; copurified with ribonuclease, which has the same molecular weight, but ribonuclease activity was removed by passage through an affinity column of agarose-5′-(4-aminophenyl phosphoryl) uridine 2′(3′) phosphate. GP43 contains about 5.9% neutral sugar, 2.3% hexosamine, and 1.6% sialic acid. A rabbit antibody to the purified GP43 reacted with human urine and serum as well as with the purified GP43. The genetic polymorphism of GP43 was then studied in desialylated human serum samples by urea-polyacrylamide gel isoelectric focusing, followed by immunoblotting with the specific antibody for GP43. Three common phenotypes, designated GP43 1, 1-2, and 2, were easily recognized using this technique and represented homozygosity or heterozygosity for two autosomal codominant alleles,GP43*1 andGP43*2. The frequencies of theGP43*1 andGP43*2 alleles in a Japanese population were 0.7683 and 0.2317, respectively. Plenum Publishing Corporation, 1989 genetic polymorphism nationallicence glycoprotein nationallicence human serum nationallicence human urine nationallicence Mizuta Keiko Department of Legal Medicine, Fukui Medical School, Matsuoka-cho, 910-11, Fukui, Japan aut Yasuda Toshihiro Department of Legal Medicine, Fukui Medical School, Matsuoka-cho, 910-11, Fukui, Japan aut Kishi Koichiro Department of Legal Medicine, Fukui Medical School, Matsuoka-cho, 910-11, Fukui, Japan aut Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 27/11-12(1989-12-01), 731-743 0006-2928 27:11-12<731 1989 27 10528 https://doi.org/10.1007/BF02396064 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02396064 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mizuta Keiko Department of Legal Medicine, Fukui Medical School, Matsuoka-cho, 910-11, Fukui, Japan aut NATIONALLICENCE 700 1- Yasuda Toshihiro Department of Legal Medicine, Fukui Medical School, Matsuoka-cho, 910-11, Fukui, Japan aut NATIONALLICENCE 700 1- Kishi Koichiro Department of Legal Medicine, Fukui Medical School, Matsuoka-cho, 910-11, Fukui, Japan aut NATIONALLICENCE 773 0- Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 27/11-12(1989-12-01), 731-743 0006-2928 27:11-12<731 1989 27 10528 NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00553993 text/html Onlinezugriff via DOI Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer