caa a22 4500 467953651 CHVBK 20180405193745.0 cr unu---uuuuu 170328e20041001xx s 000 0 eng 10.1023/B:GROW.0000049410.63154.ed doi (NATIONALLICENCE)springer-10.1023/B:GROW.0000049410.63154.ed (NATIONALLICENCE)springer-10.1007/s10725-004-2601-8 Biochemical changes during seed development in Pinus taeda L [Elektronische Daten] [Vanildo Silveira, Tiago Balbuena, Claudete Santa-Catarina, Eny Floh, Miguel Guerra, Walter Handro] Amino acids, polyamines, 3-indoleacetic acid (IAA), abscisic acid (ABA), buffer-soluble protein and starch contents and dry matter accumulation were analyzed in megagametophytes containing developing embryos during seed development in Pinus taeda. The highest total amino acids and polyamine contents occurred at the cotyledonary stage, followed by a significant decrease in the mature seed. Free polyamines exhibited higher levels than conjugated ones, with putrescine being the predominant type until the cotyledonary stage, and spermidine at the mature seed stage. IAA content increased continually from the globular stage reaching the maximum at the cotyledonary stage, followed by a decrease in the mature seed. The highest ABA level occurred at the globular stage, followed by a continuous reduction until stabilization at the pre-cotyledonary stage. Buffer-soluble protein and starch contents, and dry matter increased progressively during development, reaching their maximum values at the mature stage. Kluwer Academic Publishers, 2004 3-Indoleacetic acid nationallicence ABA nationallicence Abscisic acid nationallicence Conifer nationallicence Dry matter nationallicence Embryo development nationallicence IAA nationallicence Loblolly pine nationallicence Polyamines nationallicence Somatic embryogenesis nationallicence Starch nationallicence Storage protein nationallicence ABA : abscisic acid nationallicence CV : coefficient of variation nationallicence DM : dry matter nationallicence FM : fresh matter nationallicence HPLC : high performance liquid chromatography nationallicence IAA : 3-indoleacetic acid nationallicence Put : putrescine nationallicence Spd : spermidine nationallicence Spm : spermine nationallicence Silveira Vanildo Department of Botany, Plant Cell Biology Laboratory, IB-University of São Paulo, CP 11461, 05422-970 São, Paulo, Brasil aut Balbuena Tiago Department of Botany, Plant Cell Biology Laboratory, IB-University of São Paulo, CP 11461, 05422-970 São, Paulo, Brasil aut Santa-Catarina Claudete Department of Botany, Plant Cell Biology Laboratory, IB-University of São Paulo, CP 11461, 05422-970 São, Paulo, Brasil aut Floh Eny Department of Botany, Plant Cell Biology Laboratory, IB-University of São Paulo, CP 11461, 05422-970 São, Paulo, Brasil aut Guerra Miguel Department of Plant Science, Plant Developmental Physiology and Genetic Laboratory, Federal University of Santa Catarina, Florianópolis, Brasil aut Handro Walter Department of Botany, Plant Cell Biology Laboratory, IB-University of São Paulo, CP 11461, 05422-970 São, Paulo, Brasil aut Plant Growth Regulation Kluwer Academic Publishers 44/2(2004-10-01), 147-156 0167-6903 44:2<147 2004 44 10725 https://doi.org/10.1023/B:GROW.0000049410.63154.ed text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1023/B:GROW.0000049410.63154.ed text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Silveira Vanildo Department of Botany, Plant Cell Biology Laboratory, IB-University of São Paulo, CP 11461, 05422-970 São, Paulo, Brasil aut NATIONALLICENCE 700 1- Balbuena Tiago Department of Botany, Plant Cell Biology Laboratory, IB-University of São Paulo, CP 11461, 05422-970 São, Paulo, Brasil aut NATIONALLICENCE 700 1- Santa-Catarina Claudete Department of Botany, Plant Cell Biology Laboratory, IB-University of São Paulo, CP 11461, 05422-970 São, Paulo, Brasil aut NATIONALLICENCE 700 1- Floh Eny Department of Botany, Plant Cell Biology Laboratory, IB-University of São Paulo, CP 11461, 05422-970 São, Paulo, Brasil aut NATIONALLICENCE 700 1- Handro Walter Department of Botany, Plant Cell Biology Laboratory, IB-University of São Paulo, CP 11461, 05422-970 São, Paulo, Brasil aut NATIONALLICENCE 700 1- Guerra Miguel Department of Plant Science, Plant Developmental Physiology and Genetic Laboratory, Federal University of Santa Catarina, Florianópolis, Brasil aut NATIONALLICENCE 773 0- Plant Growth Regulation Kluwer Academic Publishers 44/2(2004-10-01), 147-156 0167-6903 44:2<147 2004 44 10725 NATIONALLICENCE 700 1- Balbuena Tiago Department of Botany, Plant Cell Biology Laboratory, IB-University of São Paulo, CP 11461, 05422-970, São Paulo, Brasil aut NATIONALLICENCE 700 1- Santa-Catarina Claudete Department of Botany, Plant Cell Biology Laboratory, IB-University of São Paulo, CP 11461, 05422-970, São Paulo, Brasil aut NATIONALLICENCE 700 1- Floh Eny Department of Botany, Plant Cell Biology Laboratory, IB-University of São Paulo, CP 11461, 05422-970, São Paulo, Brasil aut NATIONALLICENCE 856 40 https://doi.org/10.1007/s10725-004-2601-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Handro Walter Department of Botany, Plant Cell Biology Laboratory, IB-University of São Paulo, CP 11461, 05422-970, São Paulo, Brasil aut NATIONALLICENCE 700 1- Silveira Vanildo Department of Botany, Plant Cell Biology Laboratory, IB-University of São Paulo, CP 11461, 05422-970, São Paulo, Brasil aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer