caa a22 4500 467953724 CHVBK 20180405193745.0 cr unu---uuuuu 170328e20050101xx s 000 0 eng 10.1007/s10541-005-0055-x doi (NATIONALLICENCE)springer-10.1007/s10541-005-0055-x (NATIONALLICENCE)springer-10.1007/PL00021764 Biosynthesis of uridine diphosphate N-acetyl- L -fucosamine in a cell-free system from Salmonella arizonae O:59 [Elektronische Daten] [T. Druzhinina, N. Kalinchuk, V. Shibaev] The conversion of uridine diphosphate N-acetyl-D-glucosamine into uridine diphosphate N-acetyl-L-fucosamine was demonstrated with enzymes from cytoplasmic fraction of Salmonella arizonae O:59 cells in the presence of NAD+ (NADP+) and NADPH. The reaction product was identified by ion-pair, reverse-phase HPLC with the use of synthetic nucleoside diphosphate sugar standards under conditions specially developed for separation of uridine diphosphate 2-acetamido-2,6-dideoxyhexoses. L-Fucose dehydrogenase from porcine liver was shown to be applicable for determination of N-acetyl-L-fucosamine, this enzyme being used to confirm L-configuration of the amino sugar residue in the sugar nucleotide formed. MAIK "Nauka/Interperiodica”, 2005 nucleoside diphosphate sugars nationallicence 2-acetamido-2 nationallicence 6-dideoxyhexoses nationallicence uridine diphosphate N-acetyl- L -fucosamine nationallicence biosynthesis nationallicence HPLC nationallicence L -fucose dehydrogenase nationallicence NDPS : nucleoside diphosphate sugars nationallicence D -FucNAc : 2-acetamido-2,6-dideoxy-D-galactose (N-acetyl-D-fucosamine) nationallicence L -FucNAc : 2-acetamido-2,6-dideoxy-L-galactose (N-acetyl-L-fucosamine) nationallicence GlcNAc : N-acetyl-D-glucosamine nationallicence ManNAc : N-acetyl-D-mannosamine nationallicence L -PneNAc : 2-acetamido-2,6-dideoxy-L-talose (N-acetyl-L-pneumosamine) nationallicence Druzhinina T. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky pr. 47, 119991, Moscow, Russia aut Kalinchuk N. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky pr. 47, 119991, Moscow, Russia aut Shibaev V. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky pr. 47, 119991, Moscow, Russia aut Biochemistry (Moscow) Nauka/Interperiodica 70/1(2005-01-01), 85-91 0006-2979 70:1<85 2005 70 10541 https://doi.org/10.1007/s10541-005-0055-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10541-005-0055-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Druzhinina T. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky pr. 47, 119991, Moscow, Russia aut NATIONALLICENCE 700 1- Kalinchuk N. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky pr. 47, 119991, Moscow, Russia aut NATIONALLICENCE 700 1- Shibaev V. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky pr. 47, 119991, Moscow, Russia aut NATIONALLICENCE 773 0- Biochemistry (Moscow) Nauka/Interperiodica 70/1(2005-01-01), 85-91 0006-2979 70:1<85 2005 70 10541 NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00021764 text/html Onlinezugriff via DOI Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer