caa a22 4500 467953910 CHVBK 20180323165703.0 cr unu---uuuuu 170328e19880901xx s 000 0 eng 10.1007/BF01982911 doi (NATIONALLICENCE)springer-10.1007/BF01982911 (NATIONALLICENCE)springer-10.1007/BF00939316 Breeding an l -isoleucine producer by protoplast fusion of Corynebacterium glutamicum [Elektronische Daten] [Satoru Furukawa, Tomoki Azuma, Toshihide Nakanishi, Masahiro Sugimoto] Summary: Corynebacterium glutamicum R-18 is a strain forl-isoleucine production. Polyethylene glycol (PEG)-induced protoplast fusion was applied to improve the strain of thisl-isoleucine producer. Strain R-18 was fused with anl-lysine producerC. glutamicum S-37, becausel-isoleucine andl-lysine are synthesized from a common intermediate, aspartate-β-semialdehyde. Two thousand fusants were checked for their phenotypes. Most of the fusants accumulatedl-lysine, and only 0.9% of the fusants accumulatedl-isoleucine. Two strains, F-28 and F-91, were selected and cultivated in production medium. Fusant F-28 accumulated 12.1 g/l ofl-isoleucine and 4.8 g/l ofl-lysine, and fusant F-91 accumulated 4.8 g/l ofl-isoleucine and 13.0 g/l ofl-lysine, while the parental strains R-18 and S-37 accumulated 9.5 g/l ofl-isoleucine and 26.8 g/l ofl-lysine, respectively. Sugar consumption activity was improved by protoplast fusion, and thel-isoleucine production rate of F-28 was 2.4 times higher than that of R-18. Springer-Verlag, 1988 Furukawa Satoru Technical Research Laboratories, Hofu Plant, Kyowa Hakko Kogyo Co., Ltd., Kyowa-machi, 747, Hofu-shi, Yamaguchi, Japan aut Azuma Tomoki Technical Research Laboratories, Hofu Plant, Kyowa Hakko Kogyo Co., Ltd., Kyowa-machi, 747, Hofu-shi, Yamaguchi, Japan aut Nakanishi Toshihide Technical Research Laboratories, Hofu Plant, Kyowa Hakko Kogyo Co., Ltd., Kyowa-machi, 747, Hofu-shi, Yamaguchi, Japan aut Sugimoto Masahiro Technical Research Laboratories, Hofu Plant, Kyowa Hakko Kogyo Co., Ltd., Kyowa-machi, 747, Hofu-shi, Yamaguchi, Japan aut Applied Microbiology and Biotechnology Springer-Verlag 29/2-3(1988-09-01), 248-252 0175-7598 29:2-3<248 1988 29 253 https://doi.org/10.1007/BF01982911 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01982911 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Furukawa Satoru Technical Research Laboratories, Hofu Plant, Kyowa Hakko Kogyo Co., Ltd., Kyowa-machi, 747, Hofu-shi, Yamaguchi, Japan aut NATIONALLICENCE 700 1- Azuma Tomoki Technical Research Laboratories, Hofu Plant, Kyowa Hakko Kogyo Co., Ltd., Kyowa-machi, 747, Hofu-shi, Yamaguchi, Japan aut NATIONALLICENCE 700 1- Nakanishi Toshihide Technical Research Laboratories, Hofu Plant, Kyowa Hakko Kogyo Co., Ltd., Kyowa-machi, 747, Hofu-shi, Yamaguchi, Japan aut NATIONALLICENCE 700 1- Sugimoto Masahiro Technical Research Laboratories, Hofu Plant, Kyowa Hakko Kogyo Co., Ltd., Kyowa-machi, 747, Hofu-shi, Yamaguchi, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 29/2-3(1988-09-01), 248-252 0175-7598 29:2-3<248 1988 29 253 NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00939316 text/html Onlinezugriff via DOI Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer