caa a22 4500 467954224 CHVBK 20180323165703.0 cr unu---uuuuu 170328e19880901xx s 000 0 eng 10.1007/BF01982890 doi (NATIONALLICENCE)springer-10.1007/BF01982890 (NATIONALLICENCE)springer-10.1007/BF00939295 Cell adsorption control by culture conditions [Elektronische Daten] Influence of phosphate on surface properties, flocculation and adsoprtion behaviour of Corynebacterium glutamicum [Jochen Büchs, Nava Mozes, Christian Wandrey, Paul Rouxhet] Summary: Phosphate supply to growingCorynebacterium glutamicum (i.e. their phosphorus content) was found to have a distinct influence on cell wall properties, resulting in a variation of flocculation and adsorption behaviour of the bacteria. Cells saturated with phosphate are hydrophobic and show a high tendency to flocculate and to adsorb on treated glass surfaces due to hydrophobic interactions. Phosphate depletion of the cells leads to a more hydrophilic surface character and a comparatively low ability to flocculate and to adhere. The net surface charge ofC. glutamicum is not much affected by their phosphorus content. As phosphate depletion (down to a phosphorus content of about 20% of the saturation value) was shown to have no influence on growth rate and on specific 1-leucine productivity, the inducible variation of the surface properties ofC. glutamicum can be exploited to control a continuous reactor with adsorbed cells. Springer-Verlag, 1988 Büchs Jochen Institute of Biotechnology, KFA Jülich, Postbox 1913, D-5170, Jülich, Federal Republic of Germany aut Mozes Nava Unité de Chimie des Interfaces, Université Catholique de Louvain, Place Croix du Sud 1, B-1348, Louvain-la-Neuve, Belgium aut Wandrey Christian Institute of Biotechnology, KFA Jülich, Postbox 1913, D-5170, Jülich, Federal Republic of Germany aut Rouxhet Paul Unité de Chimie des Interfaces, Université Catholique de Louvain, Place Croix du Sud 1, B-1348, Louvain-la-Neuve, Belgium aut Applied Microbiology and Biotechnology Springer-Verlag 29/2-3(1988-09-01), 119-128 0175-7598 29:2-3<119 1988 29 253 https://doi.org/10.1007/BF01982890 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01982890 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Büchs Jochen Institute of Biotechnology, KFA Jülich, Postbox 1913, D-5170, Jülich, Federal Republic of Germany aut NATIONALLICENCE 700 1- Mozes Nava Unité de Chimie des Interfaces, Université Catholique de Louvain, Place Croix du Sud 1, B-1348, Louvain-la-Neuve, Belgium aut NATIONALLICENCE 700 1- Wandrey Christian Institute of Biotechnology, KFA Jülich, Postbox 1913, D-5170, Jülich, Federal Republic of Germany aut NATIONALLICENCE 700 1- Rouxhet Paul Unité de Chimie des Interfaces, Université Catholique de Louvain, Place Croix du Sud 1, B-1348, Louvain-la-Neuve, Belgium aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 29/2-3(1988-09-01), 119-128 0175-7598 29:2-3<119 1988 29 253 NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00939295 text/html Onlinezugriff via DOI Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer