caa a22 4500 467954844 CHVBK 20180323165704.0 cr unu---uuuuu 170328e19891001xx s 000 0 eng 10.1007/BF00553629 doi (NATIONALLICENCE)springer-10.1007/BF00553629 (NATIONALLICENCE)springer-10.1007/BF02396149 Comparison of soluble starch synthases and branching enzymes from leaves and kernels of normal and amylose-extender maize [Elektronische Daten] [Peter Dang, Charles Boyer] Soluble starch synthases (SS) and branching enzymes (BE) from 20-day-old maize leaves and 22-day-old seeds of normal andamylose-extender (ae) were purified by DEAE-cellulose chromatography. Elution profiles of leaf extracts showed one major SS and two BE fractions from both genotypes. The SS fractions from normal andae leaf extracts were capable of citrate-stimulated starch synthesis and had different reaction rates with various primers. The two BE fractions from normal leaf extracts differed significantly from each other but not when compared to the same BE fromae. Comparison of BE fractions fromae and normal leaves showed no differences based on chromatographic, kinetic, and immunological properties. Comparison of the leaf enzymes with endosperm enzymes showed major differences. Leaf extracts did not contain SSII or BEIIb observed in endosperm extracts. Developingae endosperm lacks BEIIb activity andae is the structural gene for BEIIb. The tissue specific expression of BEIIb in the endosperm provides the basis for explaining the tissue-specific expression ofae. We propose that as BEIIb is expressed in the endosperm, but not leaves, allelic substitution at theae locus modifies only endosperm starch synthesis. Plenum Publishing Corporation, 1989 maize nationallicence starch synthesis nationallicence endosperm nationallicence leaves nationallicence amylose-extender nationallicence branching enzyme nationallicence Dang Peter Department of Horticulture, The Pennsylvania State University, 16802, University Park, Pennsylvania aut Boyer Charles Department of Horticulture, The Pennsylvania State University, 16802, University Park, Pennsylvania aut Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 27/9-10(1989-10-01), 521-532 0006-2928 27:9-10<521 1989 27 10528 https://doi.org/10.1007/BF00553629 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00553629 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dang Peter Department of Horticulture, The Pennsylvania State University, 16802, University Park, Pennsylvania aut NATIONALLICENCE 700 1- Boyer Charles Department of Horticulture, The Pennsylvania State University, 16802, University Park, Pennsylvania aut NATIONALLICENCE 773 0- Biochemical Genetics Kluwer Academic Publishers-Plenum Publishers 27/9-10(1989-10-01), 521-532 0006-2928 27:9-10<521 1989 27 10528 NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02396149 text/html Onlinezugriff via DOI Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer