caa a22 4500 467954879 CHVBK 20180405193746.0 cr unu---uuuuu 170328e20050101xx s 000 0 eng 10.1007/PL00021770 doi (NATIONALLICENCE)springer-10.1007/PL00021770 (NATIONALLICENCE)springer-10.1007/s10541-005-0058-7 Adhesion of human neutrophils to fibronectin is inhibited by comaruman, pectin of marsh cinquefoil comarum palustre L., and by its fragments [Elektronische Daten] [S. Popov, R. Ovodova, G. Popova, I. Nikitina, Yu. Ovodov] Earlier, we detected antiinflammatory action of comaruman, pectin of the marsh cinquefoil Comarum palustre L. This effect can be explained by new data concerning inhibition of adhesion of human neutrophils to fibronectin by comaruman and its fragments. The galacturonan backbone fragment of molecular mass >10 kD appears to be the active region of the comaruman macromolecule. Comaruman CP (50-200 μg/ml) was found to decrease adhesion of neutrophils stimulated by phorbol 12-myristate 13-acetate (PMA, 1.625 μM) and by dithiothreitol (DTT, 0.5 mM). The fragments of comaruman CP-H (100 kD), CP-H1 (10-50 kD), and CP-H2 (100 kD) obtained by acidic hydrolysis and representing regions of linear polygalacturonan are shown to inhibit neutrophil adhesion more than the crude pectin. A fragment CP-E (<10 kD) obtained using pectinolysis and representing a branched region of the comaruman macromolecule failed to influence cell adhesion. The parent comaruman CP as well as fragments of its polygalacturonan backbone diminish PMA-initiated generation of oxygen radicals in neutrophils. MAIK "Nauka/Interperiodica”, 2005 pectins nationallicence Comarum palustre nationallicence comaruman nationallicence linear polygalacturonan nationallicence neutrophil adhesion nationallicence fibronectin nationallicence Popov S. Institute of Physiology, Komi Science Center, The Urals Branch of the Russian Academy of Sciences, ul. Pervomaiskaya 50, 167982, Syktyvkar, Russia aut Ovodova R. Institute of Physiology, Komi Science Center, The Urals Branch of the Russian Academy of Sciences, ul. Pervomaiskaya 50, 167982, Syktyvkar, Russia aut Popova G. Institute of Physiology, Komi Science Center, The Urals Branch of the Russian Academy of Sciences, ul. Pervomaiskaya 50, 167982, Syktyvkar, Russia aut Nikitina I. Institute of Physiology, Komi Science Center, The Urals Branch of the Russian Academy of Sciences, ul. Pervomaiskaya 50, 167982, Syktyvkar, Russia aut Ovodov Yu Institute of Physiology, Komi Science Center, The Urals Branch of the Russian Academy of Sciences, ul. Pervomaiskaya 50, 167982, Syktyvkar, Russia aut Biochemistry (Moscow) Nauka/Interperiodica 70/1(2005-01-01), 108-112 0006-2979 70:1<108 2005 70 10541 https://doi.org/10.1007/PL00021770 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00021770 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Popov S. Institute of Physiology, Komi Science Center, The Urals Branch of the Russian Academy of Sciences, ul. Pervomaiskaya 50, 167982, Syktyvkar, Russia aut NATIONALLICENCE 700 1- Ovodova R. Institute of Physiology, Komi Science Center, The Urals Branch of the Russian Academy of Sciences, ul. Pervomaiskaya 50, 167982, Syktyvkar, Russia aut NATIONALLICENCE 700 1- Popova G. Institute of Physiology, Komi Science Center, The Urals Branch of the Russian Academy of Sciences, ul. Pervomaiskaya 50, 167982, Syktyvkar, Russia aut NATIONALLICENCE 700 1- Nikitina I. Institute of Physiology, Komi Science Center, The Urals Branch of the Russian Academy of Sciences, ul. Pervomaiskaya 50, 167982, Syktyvkar, Russia aut NATIONALLICENCE 700 1- Ovodov Yu Institute of Physiology, Komi Science Center, The Urals Branch of the Russian Academy of Sciences, ul. Pervomaiskaya 50, 167982, Syktyvkar, Russia aut NATIONALLICENCE 773 0- Biochemistry (Moscow) Nauka/Interperiodica 70/1(2005-01-01), 108-112 0006-2979 70:1<108 2005 70 10541 NATIONALLICENCE 856 40 https://doi.org/10.1007/s10541-005-0058-7 text/html Onlinezugriff via DOI Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer