caa a22 4500 467955018 CHVBK 20180405193746.0 cr unu---uuuuu 170328e20050101xx s 000 0 eng 10.1007/PL00021758 doi (NATIONALLICENCE)springer-10.1007/PL00021758 (NATIONALLICENCE)springer-10.1007/s10541-005-0052-0 Construction and expression of novel immunotoxin cpIL-4(13D)-PE38KDEL with increased activity [Elektronische Daten] [J.-X. Cui, J.-F. Ji, A.-G. Lv, W.-F. Wu] Interleukin-4 receptors (IL-4Rs) are expressed on a wide variety of human cancer cells, and therefore it may be a good option to treat IL-4R-bearing tumors with IL-4-fusing immunotoxins. In this study, the gene encoding human interleukin-4 mutein cpIL-4(13D) was obtained through overlapping polymerase chain reaction. A chimeric immunotoxin was constructed by genetically fusing the mutein cpIL-4(13D) to a modified version of Pseudomonas exotoxin A (PE38KDEL) and was expressed in Escherichia coli AD494 (DE3). The expression level of the fusion protein was about 30% of the total bacterial protein assessed by SDS-PAGE analysis. After purification by affinity chromatography and anion exchange chromatography, the chimeric protein was tested for its cytotoxicity. Our data show that cpIL-4(13D)-PE38KDEL has improved cytotoxicity on IL-4R-bearing tumor cells in comparison with other IL-4-fusing immunotoxins and might be useful in treating tumors with a large number of IL-4Rs. MAIK "Nauka/Interperiodica”, 2005 immunotoxin nationallicence interleukin-4 nationallicence Pseudomonas exotoxin A nationallicence Cui J.-X Institute of Applied Ecology, Chinese Academy of Sciences, No. 72, Wenhua Road, 110016, Shenyang City, Liaoning Province, P. R. China aut Ji J.-F Beckman Research Institute, City of Hope National Medical Center, 1500 Duarte Rd. Duarte, 91010, CA, USA aut Lv A.-G Institute of Applied Ecology, Chinese Academy of Sciences, No. 72, Wenhua Road, 110016, Shenyang City, Liaoning Province, P. R. China aut Wu W.-F Institute of Applied Ecology, Chinese Academy of Sciences, No. 72, Wenhua Road, 110016, Shenyang City, Liaoning Province, P. R. China aut Biochemistry (Moscow) Nauka/Interperiodica 70/1(2005-01-01), 62-68 0006-2979 70:1<62 2005 70 10541 https://doi.org/10.1007/PL00021758 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00021758 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cui J.-X Institute of Applied Ecology, Chinese Academy of Sciences, No. 72, Wenhua Road, 110016, Shenyang City, Liaoning Province, P. R. China aut NATIONALLICENCE 700 1- Ji J.-F Beckman Research Institute, City of Hope National Medical Center, 1500 Duarte Rd. Duarte, 91010, CA, USA aut NATIONALLICENCE 700 1- Lv A.-G Institute of Applied Ecology, Chinese Academy of Sciences, No. 72, Wenhua Road, 110016, Shenyang City, Liaoning Province, P. R. China aut NATIONALLICENCE 700 1- Wu W.-F Institute of Applied Ecology, Chinese Academy of Sciences, No. 72, Wenhua Road, 110016, Shenyang City, Liaoning Province, P. R. China aut NATIONALLICENCE 773 0- Biochemistry (Moscow) Nauka/Interperiodica 70/1(2005-01-01), 62-68 0006-2979 70:1<62 2005 70 10541 NATIONALLICENCE 856 40 https://doi.org/10.1007/s10541-005-0052-0 text/html Onlinezugriff via DOI Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE NATIONALLICENCE NL-springer