caa a22 4500 467978980 CHVBK 20180323112514.0 cr unu---uuuuu 170328e19901101xx s 000 0 eng 10.1007/BF01974693 doi (NATIONALLICENCE)springer-10.1007/BF01974693 Dithiothreitol liberates non-acid labile sulfide from brain tissue of H2S-poisoned animals [Elektronische Daten] [Marcus Warenycia, Lome Goodwin, Donna Francom, Fred Dieken, Sam Kombian, R. Reiffenstein] Acid-labile sulfide measured by conventional gas dialysis and ion chromatography with electrochemical detection accounts for only a proportion of the total sulfide present in brain tissue after poisoning with NaHS, an H2S precursor. Dithiothreitol (DTT) displaced additional measurable sulfide not detectable by the conventional techniques from NaHS-poisoned brain tissue. Sulfide liberation by DTT was dose-dependent and maximal at higher DTT concentration (10 and 30 mM) and was thought to represent non-acid labile sulfide. Dithiothreitol was also found to be significantly protective against H2S poisoning. Furthermore, in vitro inhibition by sulfide of monoamine oxidase (MAO) was reversed by DTT, thus suggesting a molecular mechanism consistent with known persulfide chemistry. Persulfide formation may thus underlie some aspects of hydrogen sulfide neurotoxicity. The rational development of antidotes for use in H2S poisoning may thus have to be centered on strategies concentrating on known thiol, disulfide and persulfide chemistry. Springer-Verlag, 1990 Hydrogen sulfide nationallicence Tissue-bound sulfide nationallicence Dithiothreitol nationallicence Persulfides nationallicence Antidotal strategy nationallicence Warenycia Marcus Department of Pharmacology, University of Alberta, T6G 2H7, Edmonton, Alberta, Canada aut Goodwin Lome Alberta Environmental Centre, TOB 4L0, Vegreville, Alberta, Canada aut Francom Donna Alberta Environmental Centre, TOB 4L0, Vegreville, Alberta, Canada aut Dieken Fred Alberta Environmental Centre, TOB 4L0, Vegreville, Alberta, Canada aut Kombian Sam Department of Pharmacology, University of Alberta, T6G 2H7, Edmonton, Alberta, Canada aut Reiffenstein R. Department of Pharmacology, University of Alberta, T6G 2H7, Edmonton, Alberta, Canada aut Archives of Toxicology Springer-Verlag 64/8(1990-11-01), 650-655 0340-5761 64:8<650 1990 64 204 https://doi.org/10.1007/BF01974693 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01974693 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Warenycia Marcus Department of Pharmacology, University of Alberta, T6G 2H7, Edmonton, Alberta, Canada aut NATIONALLICENCE 700 1- Goodwin Lome Alberta Environmental Centre, TOB 4L0, Vegreville, Alberta, Canada aut NATIONALLICENCE 700 1- Francom Donna Alberta Environmental Centre, TOB 4L0, Vegreville, Alberta, Canada aut NATIONALLICENCE 700 1- Dieken Fred Alberta Environmental Centre, TOB 4L0, Vegreville, Alberta, Canada aut NATIONALLICENCE 700 1- Kombian Sam Department of Pharmacology, University of Alberta, T6G 2H7, Edmonton, Alberta, Canada aut NATIONALLICENCE 700 1- Reiffenstein R. Department of Pharmacology, University of Alberta, T6G 2H7, Edmonton, Alberta, Canada aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 64/8(1990-11-01), 650-655 0340-5761 64:8<650 1990 64 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer