caa a22 4500 467979065 CHVBK 20180323112514.0 cr unu---uuuuu 170328e19900701xx s 000 0 eng 10.1007/BF01973460 doi (NATIONALLICENCE)springer-10.1007/BF01973460 Combined effect of cadmium (CdCl2) and high temperature on HeLa S3 cells [Elektronische Daten] [Takuji Kishimoto, Yoichiro Fukuzawa, Manabu Tada] The combined effect of cadmium (CdCl2) and high temperature on the subsequent growth, the synthesis of nucleic acids and protein, and the cell cycle in HeLa S3 cells was investigated. The subsequent growth rate was depressed by cadmium at 37.1°C. This suppression effect was enhanced at 40.4° C compared with the effect at 37.1° C. The synthesis of DNA, RNA and protein was inhibited at higher temperatures in cultivation without cadmium, and depressed at each temperature in a concentration-dependent manner by the addition of cadmium. The DNA, RNA and protein-IC50 values at 40.4° C decreased compared with the values at 37.1° C. The DNA and RNA-IC50 values were significantly decreased (p<0.01,p<0.005, respectively) depending on the temperature. After treating the cells with cadmium at 40.4° C, the DNA/BrdUrd distribution showed that the rate of dead cells increased and the rate of the G1/G0 phase decreased. These results indicate that the inhibitory effect of cadmium on the subsequent growth of HeLa S3 cells is enhanced at high temperature and this enhancement is related to the increased inhibitory effect of cadmium on DNA and RNA synthesis at high temperature. Springer-Verlag, 1990 Cadmium nationallicence High temperature nationallicence HeLa S3 cell nationallicence Nucleic acid synthesis nationallicence Cell cycle nationallicence Kishimoto Takuji Department of Environmental Medicine, Shimane Medical University, 693, Izumo, Japan aut Fukuzawa Yoichiro Department of Environmental Medicine, Shimane Medical University, 693, Izumo, Japan aut Tada Manabu Department of Environmental Medicine, Shimane Medical University, 693, Izumo, Japan aut Archives of Toxicology Springer-Verlag 64/5(1990-07-01), 383-386 0340-5761 64:5<383 1990 64 204 https://doi.org/10.1007/BF01973460 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01973460 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kishimoto Takuji Department of Environmental Medicine, Shimane Medical University, 693, Izumo, Japan aut NATIONALLICENCE 700 1- Fukuzawa Yoichiro Department of Environmental Medicine, Shimane Medical University, 693, Izumo, Japan aut NATIONALLICENCE 700 1- Tada Manabu Department of Environmental Medicine, Shimane Medical University, 693, Izumo, Japan aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 64/5(1990-07-01), 383-386 0340-5761 64:5<383 1990 64 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer