caa a22 4500 467979103 CHVBK 20180323112514.0 cr unu---uuuuu 170328e19900701xx s 000 0 eng 10.1007/BF01973464 doi (NATIONALLICENCE)springer-10.1007/BF01973464 Hepatotoxin-induced hypertaurinuria: a proton NMR study [Elektronische Daten] [Susan Sanins, Jeremy Nicholson, Cliff Elcombe, John Timbrell] The urinary excretion of taurine by rats after dosing with various hepatotoxins has been investigated by1H NMR spectroscopy. After single hepatotoxic doses of hydrazine, carbon tetrachloride, 1-naphthylisothiocyanate, or thioacetamide there was biochemical and histopathological evidence of hepatic damage. Proton NMR spectroscopy of the urine collected for 24 h after dosing from these animals revealed a marked elevation in taurine (control 11.9 μmole/h/kg) after dosing with thioacetamide (42.2 μmole/h/kg), carbon tetrachloride (52.5 gmmole/ h/kg), 1-naphthyl-isothiocyanate (80.4 μmole/h/kg) and hydrazine (52.9 μmmole/h/kg). After allyl alcohol administration there was no increase in taurine excretion (7.5 μmol/h/kg). The excretion of taurine after hydrazine administration was dose related. High resolution proton NMR spectroscopic analysis of urine also revealed resonances from several metabolites of hydrazine, an N-acetylcysteine conjugate of allyl alcohol, and acetamide as a metabolite of thioacetamide after dosing with the respective compounds. Changes in endogenous substances that may be related to the pathological events were also detected, such as a decrease in the excretion of 2-oxoglutarate and citrate after both hydrazine and carbon tetrachloride administration. The results confirm that proton NMR spectroscopic analysis of urine is a powerful analytical tool for the evaluation and study of toxic substances. Furthermore, measurement of urinary taurine may provide a non-invasive indicator of acute hepatic damage with certain classes of hepatotoxins. Springer-Verlag, 1990 Hepatotoxins nationallicence Hypertaurinuria nationallicence Proton NMR studies nationallicence Rats nationallicence Sanins Susan Chemistry Department, Birkbeck College, University of London, London, UK aut Nicholson Jeremy Chemistry Department, Birkbeck College, University of London, London, UK aut Elcombe Cliff Central Toxicology Laboratory, ICI PLC, University of London, London, UK aut Timbrell John Toxicology Unit, School of Pharmacy, University of London, London, UK aut Archives of Toxicology Springer-Verlag 64/5(1990-07-01), 407-411 0340-5761 64:5<407 1990 64 204 https://doi.org/10.1007/BF01973464 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01973464 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sanins Susan Chemistry Department, Birkbeck College, University of London, London, UK aut NATIONALLICENCE 700 1- Nicholson Jeremy Chemistry Department, Birkbeck College, University of London, London, UK aut NATIONALLICENCE 700 1- Elcombe Cliff Central Toxicology Laboratory, ICI PLC, University of London, London, UK aut NATIONALLICENCE 700 1- Timbrell John Toxicology Unit, School of Pharmacy, University of London, London, UK aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 64/5(1990-07-01), 407-411 0340-5761 64:5<407 1990 64 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer