caa a22 4500 467979146 CHVBK 20180323112514.0 cr unu---uuuuu 170328e19900701xx s 000 0 eng 10.1007/BF01973456 doi (NATIONALLICENCE)springer-10.1007/BF01973456 Immunoassays for proteins alkylated by nicotine-derived N-nitrosamines [Elektronische Daten] [Brian Talbot, Serge Desnoyers, Andre Castonguay] Polyclonal antibodies recognizing the pyridyloxobutyl (POB) moiety of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) were produced in rabbits immunized either with POB-bovine albumin or POB-Sepharose. The POB intermediates necessary to modify the protein were generated by alkaline (pH 9.0) treatment of the synthetic precursor 4-(carbethoxynitrosamino)-1-(3-pyridyl)-1-butanone. In a competitive enzyme linked immunoabsorbent assay (ELISA), 70 pmole NNK inhibited 50% of the binding of the anti-POB antibodies to POB-protein absorbed on microtiterplates. This 50% inhibition varied from 70 pmole to 200 nmole using a series of NNK analogues, depending on the integrity of the POB moiety. Immunological techniques initiated in this study detect NNK-protein conjugates or measure the quantity of POB groups liberated upon alkaline or acid treatment of NNK modified protein. Springer-Verlag, 1990 Carcinogen-protein adducts nationallicence Tobacco-specific N-nitrosamines nationallicence Dosimeter of carcinogen exposure nationallicence Immunoassay nationallicence NNN : N′-nitrosonomicotine nationallicence NNK : 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone nationallicence NNK-N-oxide : 4-(methylnitrosamino)-1-(3-pyridyl-N-oxide)-1-butanone nationallicence NNAL : 4-(methylnitrosamino)-1-(3-pyridyl) butan-1-ol nationallicence ELISA : Enzyme linked immunosorbent assay nationallicence SDS : Sodium dodecyl sulfate nationallicence POB : pyridyloxobutyl nationallicence PBS : phosphate buffered saline nationallicence Talbot Brian Department of Biology, Faculty of Sciences, University of Sherbrooke, J1K 2R1, Sherbrooke, Québec, Canada aut Desnoyers Serge Department of Biology, Faculty of Sciences, University of Sherbrooke, J1K 2R1, Sherbrooke, Québec, Canada aut Castonguay Andre Laboratory of Cancer Etiology and Chemoprevention, School of Pharmacy, Laval University, G1K 7P4, Quebec Q. C, Canada aut Archives of Toxicology Springer-Verlag 64/5(1990-07-01), 360-364 0340-5761 64:5<360 1990 64 204 https://doi.org/10.1007/BF01973456 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01973456 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Talbot Brian Department of Biology, Faculty of Sciences, University of Sherbrooke, J1K 2R1, Sherbrooke, Québec, Canada aut NATIONALLICENCE 700 1- Desnoyers Serge Department of Biology, Faculty of Sciences, University of Sherbrooke, J1K 2R1, Sherbrooke, Québec, Canada aut NATIONALLICENCE 700 1- Castonguay Andre Laboratory of Cancer Etiology and Chemoprevention, School of Pharmacy, Laval University, G1K 7P4, Quebec Q. C, Canada aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 64/5(1990-07-01), 360-364 0340-5761 64:5<360 1990 64 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer