caa a22 4500 467979197 CHVBK 20180323112514.0 cr unu---uuuuu 170328e19900301xx s 000 0 eng 10.1007/BF01974391 doi (NATIONALLICENCE)springer-10.1007/BF01974391 Changes in muntjac fibroblasts associated with the acquisition of cadmium resistance [Elektronische Daten] A pre-resistance, transitional and post-resistance study [Muriel Ord, Rakesh Chibber, Simon Bouffler, Trevor Courtney] A series of cell lines with different levels of resistance to continuous cadmium exposure has been developed from an immortal but non-transformed muntjac fibroblast cell line. Concentrations accepted in their culture medium range from 0.1 μM for the cadmium sensitive parent line to 5 μM for the intermediate "cadmium-tolerant” line, to 5, 10, 20 and 50 μM for the four "cadmium-resistant” lines. The present paper follows the morphological changes which accompanied the development of resistance through a 20-month pre-resistance period, a relatively abrupt 6-week transitional period and a 3-year post-resistance period, during which time levels of cadmium resistance were increased. Initial changes which led to the cadmium-tolerant CR5 cell line included (i) increased efficiency in autophagocytosing damaged cell components and in ridding the cell of residual waste materials, (ii) a reduction in fluid filled vacuoles and (iii) improved recycling and/or replacement of cadmium-damaged cell membrane. With the advent of cadmium resistance the intracellular damage necessitating these activities disappeared, yet the series of changes which occurred included a massive build-up of Golgi and the appearance of a trans-Golgi tubular network in addition to cytoskeletal and membrane changes. Though metallothionein levels are greater in the cadmium-resistant variants, their increase appears inadequate on their own to account for the high levels of resistance. The post-resistance changes which accompanied each step-up in cadmium resistance included further membrane and glycocalyx changes, in addition to continued increases in Golgi bodies and tubular network. This paper details the morphological changes which occurred throughout the 5-year period, tests the direct dependence of each on the presence of cadmium and examines their possible contribution to a cadmium protective mechanism. Springer-Verlag, 1990 Cadmium nationallicence Resistance nationallicence Transformation nationallicence Golgi complex nationallicence Cytoskeleton nationallicence Ord Muriel Biology Department, Southampton University, Bassett Crescent East, SO9 3TU, Southampton, UK aut Chibber Rakesh Biology Department, Southampton University, Bassett Crescent East, SO9 3TU, Southampton, UK aut Bouffler Simon CRC Mammalian Cell DNA Repair Research Group, Zoology Department, Cambridge University, Downing Street, CB2 3EJ, Cambridge, UK aut Courtney Trevor Biology Department, Southampton University, Bassett Crescent East, SO9 3TU, Southampton, UK aut Archives of Toxicology Springer-Verlag 64/2(1990-03-01), 77-90 0340-5761 64:2<77 1990 64 204 https://doi.org/10.1007/BF01974391 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01974391 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ord Muriel Biology Department, Southampton University, Bassett Crescent East, SO9 3TU, Southampton, UK aut NATIONALLICENCE 700 1- Chibber Rakesh Biology Department, Southampton University, Bassett Crescent East, SO9 3TU, Southampton, UK aut NATIONALLICENCE 700 1- Bouffler Simon CRC Mammalian Cell DNA Repair Research Group, Zoology Department, Cambridge University, Downing Street, CB2 3EJ, Cambridge, UK aut NATIONALLICENCE 700 1- Courtney Trevor Biology Department, Southampton University, Bassett Crescent East, SO9 3TU, Southampton, UK aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 64/2(1990-03-01), 77-90 0340-5761 64:2<77 1990 64 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer