caa a22 4500 467979200 CHVBK 20180323112514.0 cr unu---uuuuu 170328e19900301xx s 000 0 eng 10.1007/BF01974396 doi (NATIONALLICENCE)springer-10.1007/BF01974396 Nuclear aberrations in hair follicle cells of patients receiving cyclophosphamide [Elektronische Daten] A possible in vivo assay for human exposure to genotoxic agents [Mark Goldberg, Linda Tackaberry, Margaret Hardy, John Noseworthy] The toxic effect of cyclophosphamide on the proliferative cell population of hair follicles plucked from the human scalp was examined by the in vivo nuclear aberration assay. Patients participating in an independent clinical trial received oral low dose cyclophosphamide, intravenous high dose cyclophosphamide or oral placebo treatment. The percent of cells with nuclear aberrations (indicating apoptosis, a special form of cell death) and the percent of mitotic cells, in the hair matrix, were calculated for each patient before treatment and at several time points following cyclophosphamide or placebo treatment. The mean percentages of nuclear aberrations in both the treated Low dose and High dose cyclophosphamide patients were significantly higher than those for the pre-treatment and Placebo patients. The nuclear aberrations in hair follicle cells increased from pre-treatment (and Placebo) to treated Low dose and finally to treated High dose patients. The average percentage for pre-treatment samples from all patients was 0.06 ±0.03 SE. For 1 week and 1 month samples from Low dose patients it was 0.35 ±0.08 SE, and for combined 2,3 and 4 day samples from High dose patients it was 1.08 ±0.12 SE. Cyclophosphamide also had a significant effect on mitosis. A decrease in mitotic activity was observed at 1 month following the initial low dose cyclophosphamide treatment and at 24±2 h following each of the first two high dose cyclophosphamide treatments. The observed increase in nuclear aberrations following low dose as well as high dose cyclophosphamide suggests that it is feasible to use the nuclear aberration assay for in vivo human genotoxicity testing, using proliferating hair follicle cells. Springer-Verlag, 1990 Cyclophosphamide nationallicence Nuclear aberration assay nationallicence Hair follicles nationallicence Human genotoxicity nationallicence Apoptosis nationallicence Goldberg Mark Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut Tackaberry Linda Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut Hardy Margaret Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut Noseworthy John Department of Clinical Neurological Sciences, University Hospital, Postal Stn. A, P. O. Box 5339, N6A 5A5, London, Ontario, Canada aut Archives of Toxicology Springer-Verlag 64/2(1990-03-01), 116-121 0340-5761 64:2<116 1990 64 204 https://doi.org/10.1007/BF01974396 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01974396 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Goldberg Mark Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut NATIONALLICENCE 700 1- Tackaberry Linda Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut NATIONALLICENCE 700 1- Hardy Margaret Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, N1G 2W1, Guelph, Ontario, Canada aut NATIONALLICENCE 700 1- Noseworthy John Department of Clinical Neurological Sciences, University Hospital, Postal Stn. A, P. O. Box 5339, N6A 5A5, London, Ontario, Canada aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 64/2(1990-03-01), 116-121 0340-5761 64:2<116 1990 64 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer