caa a22 4500 467979219 CHVBK 20180323112514.0 cr unu---uuuuu 170328e19900301xx s 000 0 eng 10.1007/BF01974394 doi (NATIONALLICENCE)springer-10.1007/BF01974394 Effects of diethyl maleate (DEM), a glutathione depletor, on prostaglandin synthesis in the isolated perfused spleen of rabbits [Elektronische Daten] [Toshihiro Hidaka, Hiroaki Furuno, Takahiro Inokuchi, Ryohei Ogura] To investigate the role of glutathione (GSH) on prostaglandin (PG) synthesis, isolated rabbit spleens were perfused with Tyrode's solution with or without the addition of diethyl maleate (DEM) in concentrations up to 1 mM. In the absence of DEM, PG synthesis was stimulated by the Ca2+ ionophore A23187 (20 nmole) or arachidonate (0.4 μmole). Prostaglandin (PG) E2 was a major product, accounting for 60-70% of the total cyclooxygenase products. Small amounts of PGF2α, 6-keto-PGF1α, PGD2 and thromboxane (Tx) B2 were also produced. When DEM was added to the perfusion medium, GSH content decreased dose-dependently with increasing DEM concentration. Lactate dehydrogenase activity was not detected in the venous effluent, indicating that DEM depleted intrasplenic GSH without causing any lysis of cellular membranes. A23187-induced production of PGs and of Tx was decreased with increasing concentrations of DEM up to 0.5 mM, whereas at 1.0 mM DEM, these products showed a tendency to increase as compared with levels at 0.5 mM DEM. However, this increase was only significant for TxB2, which returned to levels obtained in the absence of DEM. DEM 1 mM did not cause cell lysis, but it appears to perturb the cell membrane to a degree similar to that which occurs with stimulation of phospholipase A2. The small but significant increase of TxB2 with 1.0 mM DEM could be a result of decreased PGE2 isomerase activity. Perfusion with arachidonate gave virtually identical results: 1.0 mM DEM attenuated the production of all prostanoids except for TxB2 as compared with untreated controls. These results suggest that GSH contributes to the regulation and/or maintenance of PGs synthesis. Springer-Verlag, 1990 Prostaglandins nationallicence Thromboxane nationallicence Spleen nationallicence Glutathione nationallicence Diethyl maleate nationallicence Hidaka Toshihiro Department of Medical Biochemistry, Kurume University School of Medicine, Asahimachi 67, Kurume, 830, Fukuoka, Japan aut Furuno Hiroaki Department of Medical Biochemistry, Kurume University School of Medicine, Asahimachi 67, Kurume, 830, Fukuoka, Japan aut Inokuchi Takahiro Institute of Medical Mass Spectrometry, Kurume University School of Medicine, Asahimachi 67, Kurume, 830, Fukuoka, Japan aut Ogura Ryohei Department of Medical Biochemistry, Kurume University School of Medicine, Asahimachi 67, Kurume, 830, Fukuoka, Japan aut Archives of Toxicology Springer-Verlag 64/2(1990-03-01), 103-108 0340-5761 64:2<103 1990 64 204 https://doi.org/10.1007/BF01974394 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01974394 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hidaka Toshihiro Department of Medical Biochemistry, Kurume University School of Medicine, Asahimachi 67, Kurume, 830, Fukuoka, Japan aut NATIONALLICENCE 700 1- Furuno Hiroaki Department of Medical Biochemistry, Kurume University School of Medicine, Asahimachi 67, Kurume, 830, Fukuoka, Japan aut NATIONALLICENCE 700 1- Inokuchi Takahiro Institute of Medical Mass Spectrometry, Kurume University School of Medicine, Asahimachi 67, Kurume, 830, Fukuoka, Japan aut NATIONALLICENCE 700 1- Ogura Ryohei Department of Medical Biochemistry, Kurume University School of Medicine, Asahimachi 67, Kurume, 830, Fukuoka, Japan aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 64/2(1990-03-01), 103-108 0340-5761 64:2<103 1990 64 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer