caa a22 4500 46797926X CHVBK 20180323112515.0 cr unu---uuuuu 170328e19900301xx s 000 0 eng 10.1007/BF01974397 doi (NATIONALLICENCE)springer-10.1007/BF01974397 Possible role of the acetone-inducible cytochrome P-450IIE1 in the metabolism and hepatotoxicity of thiobenzamide [Elektronische Daten] [E. Chieli, M. Saviozzi, P. Puccini, V. Longo, P. Gervasi] The effect of acetone pretreatment (5% in drinking water for 10 days on rat liver metabolism and toxicity of thiobenzamide (TB) was investigated. Hepatic microsomes from acetone-pretreated rats showed a significant increase of TB-S-oxidation rate which, on the basis of selective thermal inactivation of FAD-containing monooxygenase (FADM), appeared dependent only on cytochrome P-450. Furthermore, TB was able to competitively inhibit acetone hydroxylase (AcH), an enzymatic reaction highly specific for the P-450IIE1 isozyme. Acetone pretreatment of rats also produced an exacerbation of liver damage induced by acute administration of TB (150 mg/ kg), as judged by the extent of liver necrosis and serum alanine-amino transferase (ALAT) activities. Coadministration of acetone with TB reduced on the other hand the extent of liver damage. The findings suggest that P-450 species induced by acetone, and in particular the P-450IIE1 isozyme, could be involved in the biotransformation of TB. Springer-Verlag, 1990 Thiobenzamide nationallicence Acetone nationallicence Cytochrome P-450IIE1 nationallicence Hepatotoxicity nationallicence Chieli E. Scuola Medica, Istituto di Patologia Generale dell'Università di Pisa, via Roma 55, I-56126, Pisa, Italy aut Saviozzi M. Scuola Medica, Istituto di Patologia Generale dell'Università di Pisa, via Roma 55, I-56126, Pisa, Italy aut Puccini P. Istituto di Mutagenesi e Differenziamento, CNR, via Svezia 10, I-56124, Pisa, Italy aut Longo V. Istituto di Mutagenesi e Differenziamento, CNR, via Svezia 10, I-56124, Pisa, Italy aut Gervasi P. Istituto di Mutagenesi e Differenziamento, CNR, via Svezia 10, I-56124, Pisa, Italy aut Archives of Toxicology Springer-Verlag 64/2(1990-03-01), 122-127 0340-5761 64:2<122 1990 64 204 https://doi.org/10.1007/BF01974397 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01974397 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chieli E. Scuola Medica, Istituto di Patologia Generale dell'Università di Pisa, via Roma 55, I-56126, Pisa, Italy aut NATIONALLICENCE 700 1- Saviozzi M. Scuola Medica, Istituto di Patologia Generale dell'Università di Pisa, via Roma 55, I-56126, Pisa, Italy aut NATIONALLICENCE 700 1- Puccini P. Istituto di Mutagenesi e Differenziamento, CNR, via Svezia 10, I-56124, Pisa, Italy aut NATIONALLICENCE 700 1- Longo V. Istituto di Mutagenesi e Differenziamento, CNR, via Svezia 10, I-56124, Pisa, Italy aut NATIONALLICENCE 700 1- Gervasi P. Istituto di Mutagenesi e Differenziamento, CNR, via Svezia 10, I-56124, Pisa, Italy aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 64/2(1990-03-01), 122-127 0340-5761 64:2<122 1990 64 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer