caa a22 4500 467979286 CHVBK 20180323112515.0 cr unu---uuuuu 170328e19900301xx s 000 0 eng 10.1007/BF01974393 doi (NATIONALLICENCE)springer-10.1007/BF01974393 Biomonitoring of aromatic amines III: Hemoglobin binding of benzidine and some benzidine congeners [Elektronische Daten] [G. Birner, W. Albrecht, H. Neumann] Covalent binding of benzidine and some congeners to hemoglobin was studied in female Wistar rats after oral administration. Hemoglobin adducts were hydrolyzed under alkaline conditions, and the arylamines extracted and analysed by HPLC with electrochemical detector. With benzidine, three cleavage products were observed, the major component being monoacetylbenzidine. This indicates that 4-nitroso-4′-N-acetylaminobiphenyl is the major reactive metabolite in erythrocytes. In addition benzidine and 4-aminobiphenyl were identified. The latter indicates a hitherto unknown metabolic pathway of benzidine. With 3,3′-dichlorobenzidine-dihydrochloride, 3,3′-dimethoxybenzidine and 3,3′-dimethylbenzidine two cleavage products were observed, the parent diamines being present in excess to or in amounts comparable to the monoacetyl derivative. With 3,3′,5,5′-tetramethylbenzidine a hemoglobin adduct could not be found. When the azo dye direct red 28 was administered to the animals, the three cleavage products typical for benzidine were found, indicating that benzidine became bioavailable after reductive cleavage of the azo compound. In this case the fraction of 4-aminobiphenyl was greater than after benzidine. It is proposed to use the analysis of hemoglobin adducts in human blood to control the exposure of individuals to these carcinogenic chemicals in the course of biochemical effect monitoring. Springer-Verlag, 1990 Biological monitoring nationallicence Hemoglobin binding nationallicence 4-Aminobiphenyl nationallicence Benzidine nationallicence 3,3′-Dichlorobenzidine nationallicence 3,3′-Dimethoxybenzidine nationallicence 3,3′-Dimethylbenzidine nationallicence 3,3′,5,5′-Tetramethylbenzidine nationallicence Direct red 28 nationallicence Exposure control nationallicence Risk assessment nationallicence Birner G. Institute of Pharmacology and Toxicology, University of Würzburg, Versbacher Strasse 9, D-8700, Würzburg, Federal Republic of Germany aut Albrecht W. Institute of Pharmacology and Toxicology, University of Würzburg, Versbacher Strasse 9, D-8700, Würzburg, Federal Republic of Germany aut Neumann H. Institute of Pharmacology and Toxicology, University of Würzburg, Versbacher Strasse 9, D-8700, Würzburg, Federal Republic of Germany aut Archives of Toxicology Springer-Verlag 64/2(1990-03-01), 97-102 0340-5761 64:2<97 1990 64 204 https://doi.org/10.1007/BF01974393 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01974393 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Birner G. Institute of Pharmacology and Toxicology, University of Würzburg, Versbacher Strasse 9, D-8700, Würzburg, Federal Republic of Germany aut NATIONALLICENCE 700 1- Albrecht W. Institute of Pharmacology and Toxicology, University of Würzburg, Versbacher Strasse 9, D-8700, Würzburg, Federal Republic of Germany aut NATIONALLICENCE 700 1- Neumann H. Institute of Pharmacology and Toxicology, University of Würzburg, Versbacher Strasse 9, D-8700, Würzburg, Federal Republic of Germany aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 64/2(1990-03-01), 97-102 0340-5761 64:2<97 1990 64 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 464667763 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer