caa a22 4500 467983313 CHVBK 20180323112524.0 cr unu---uuuuu 170328e19900501xx s 000 0 eng 10.1007/BF00210800 doi (NATIONALLICENCE)springer-10.1007/BF00210800 Construction and characterization of band-specific DNA libraries [Elektronische Daten] [Hermann-Josef Lüdecke, Gabriele Senger, Uwe Claussen, Bernhard Horsthemke] Summary: A universally primed polymerase chain reaction was developed to amplify DNA dissected from GTG-banded human chromosomes. The amplification products are cloned into plasmid vectors, which allow the rapid characterization of recombinant clones. Starting from 20-40 chromosome fragments, several thousand independent clones detecting single-copy sequences can be obtained. Although these libraries comprise only a few percent of the dissected DNA, they provide narrowly spaced anchor clones for the molecular characterization of chromosome bands and the identification of gene sequences. Here we describe the construction and characterization of DNA libraries for the Langer-Giedion syndrome chromosome region (LGCR, 8q23-24.1), Wilms tumor chromosome region 1 (WT1, 11p13), Prader-Willi syndrome/Angelman syndrome chromosome region (PWCR/ANCR, 15q11.2-12), meningioma chromosome region (MGCR, 22q12-13), and fragile X chromosome region (FRAXA, Xq27.3). Springer-Verlag, 1990 Lüdecke Hermann-Josef Institut für Humangenetik, Universitätsklinikum, Hufelandstrasse 55, D-4300, Essen 1, Germany aut Senger Gabriele Institut für Humangenetik der Universität, Schwabachanlage 10, D-8520, Erlangen, Germany aut Claussen Uwe Institut für Humangenetik der Universität, Schwabachanlage 10, D-8520, Erlangen, Germany aut Horsthemke Bernhard Institut für Humangenetik, Universitätsklinikum, Hufelandstrasse 55, D-4300, Essen 1, Germany aut Human Genetics Springer-Verlag 84/6(1990-05-01), 512-516 0340-6717 84:6<512 1990 84 439 https://doi.org/10.1007/BF00210800 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00210800 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lüdecke Hermann-Josef Institut für Humangenetik, Universitätsklinikum, Hufelandstrasse 55, D-4300, Essen 1, Germany aut NATIONALLICENCE 700 1- Senger Gabriele Institut für Humangenetik der Universität, Schwabachanlage 10, D-8520, Erlangen, Germany aut NATIONALLICENCE 700 1- Claussen Uwe Institut für Humangenetik der Universität, Schwabachanlage 10, D-8520, Erlangen, Germany aut NATIONALLICENCE 700 1- Horsthemke Bernhard Institut für Humangenetik, Universitätsklinikum, Hufelandstrasse 55, D-4300, Essen 1, Germany aut NATIONALLICENCE 773 0- Human Genetics Springer-Verlag 84/6(1990-05-01), 512-516 0340-6717 84:6<512 1990 84 439 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer