caa a22 4500 467983348 CHVBK 20180323112524.0 cr unu---uuuuu 170328e19900501xx s 000 0 eng 10.1007/BF00210799 doi (NATIONALLICENCE)springer-10.1007/BF00210799 Microdissection of banded human chromosomes [Elektronische Daten] [Gabriele Senger, Hermann-Josef Lüdecke, Bernhard Horsthemke, Uwe Claussen] Summary: Physical dissection of metaphase chromosomes is the most straightforward approach for the isolation of DNA sequences from specific chromosome regions. However, conventional microdissection techniques are too crude and inefficient for analysis of the human genome. Here we describe a technique for the precise dissection of single bands from GTG-banded chromosomes. Cells from normal amniotic fluid cell cultures are harvested by the pipette method. Microdissection is performed on an inverted microscope (magnification 1250 x) with the help of extended siliconized glass needles and an electronically controlled micromanipulator. Enzymatic amplification of the dissected DNA allows the construction of band-specific DNA libraries from as few as 20 dissected chromosome fragments. Springer-Verlag, 1990 Senger Gabriele Institut für Humangenetik der Universität, Schwabachanlage 10, D-8520, Erlangen, Germany aut Lüdecke Hermann-Josef Institut für Humangenetik, Universitätsklinikum, Hufelandstrasse 55, D-4300, Essen 1, Germany aut Horsthemke Bernhard Institut für Humangenetik, Universitätsklinikum, Hufelandstrasse 55, D-4300, Essen 1, Germany aut Claussen Uwe Institut für Humangenetik der Universität, Schwabachanlage 10, D-8520, Erlangen, Germany aut Human Genetics Springer-Verlag 84/6(1990-05-01), 507-511 0340-6717 84:6<507 1990 84 439 https://doi.org/10.1007/BF00210799 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00210799 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Senger Gabriele Institut für Humangenetik der Universität, Schwabachanlage 10, D-8520, Erlangen, Germany aut NATIONALLICENCE 700 1- Lüdecke Hermann-Josef Institut für Humangenetik, Universitätsklinikum, Hufelandstrasse 55, D-4300, Essen 1, Germany aut NATIONALLICENCE 700 1- Horsthemke Bernhard Institut für Humangenetik, Universitätsklinikum, Hufelandstrasse 55, D-4300, Essen 1, Germany aut NATIONALLICENCE 700 1- Claussen Uwe Institut für Humangenetik der Universität, Schwabachanlage 10, D-8520, Erlangen, Germany aut NATIONALLICENCE 773 0- Human Genetics Springer-Verlag 84/6(1990-05-01), 507-511 0340-6717 84:6<507 1990 84 439 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer