caa a22 4500 467983437 CHVBK 20180323112525.0 cr unu---uuuuu 170328e19900501xx s 000 0 eng 10.1007/BF00210801 doi (NATIONALLICENCE)springer-10.1007/BF00210801 Cocultivation of Fanconi anemia cells and of mouse lymphoma mutants leads to interspecies complementation of chromosomal hypersensitivity to DNA cross-linking agents [Elektronische Daten] [Filippo Rosselli, Ethel Moustacchi] Summary: We have studied the effects of cocultivation on the frequency of mitomycin C (MMC)-induced chromosomal aberrations. This was carried out by cocultivating Fanconi anemia (FA) cells from the genetic complementation groups A and B with both normal mouse lymphoma L5178Y cells and the derived "FA-like” mutant cells, MCN-151 and MCE-50, assigned to complementation groups I and II, respectively. The results show a partial complementation of the defect (i.e. a reduction in the frequency of chromosomal aberration) in FA group A cells cocultured with normal or group II mouse cells, and a partial correction of mouse group I cells cocultived with normal or FA group B human cells. No reciprocal effects were observed between FA group A cells and mouse group I mutant cells; the frequencies of MMC-induced chromosomal aberrations in these cells remained unchanged by cocultivation. Moreover, no complementation was observed for both FA group B cells and mouse group II cells, after cocultivation with normal cells of either mouse or human origin. This implies that a diffusible factor released by normal human and mouse cells, and by FA group B and mouse group II mutant cells, can correct at least in part the chromosomal defect of FA group A and mouse group I mutant cells. With normal human or mouse cells, the frequency of chromosomal breakage after cocultivation remains the same as that observed in non-cocultived cells. This suggests that no detectable clastogenic factor is released by human FA or "FA-like” mouse cells. Springer-Verlag, 1990 Rosselli Filippo Dottorato in Scienze Genetiche, Università di Ferrara, Istituto di Zoologia, 46, Via Borsari, Ferrara, Italy aut Moustacchi Ethel Institut Curie — Section de Biologie, 26 Rue d'Ulm, 05, Paris Cedex, France aut Human Genetics Springer-Verlag 84/6(1990-05-01), 517-521 0340-6717 84:6<517 1990 84 439 https://doi.org/10.1007/BF00210801 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00210801 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rosselli Filippo Dottorato in Scienze Genetiche, Università di Ferrara, Istituto di Zoologia, 46, Via Borsari, Ferrara, Italy aut NATIONALLICENCE 700 1- Moustacchi Ethel Institut Curie — Section de Biologie, 26 Rue d'Ulm, 05, Paris Cedex, France aut NATIONALLICENCE 773 0- Human Genetics Springer-Verlag 84/6(1990-05-01), 517-521 0340-6717 84:6<517 1990 84 439 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer