caa a22 4500 46798350X CHVBK 20180323112525.0 cr unu---uuuuu 170328e19900201xx s 000 0 eng 10.1007/BF00200574 doi (NATIONALLICENCE)springer-10.1007/BF00200574 Analysis of the transgenome of MET transfectant cell lines reveals that MET activation is accompanied by an interstitial insertion [Elektronische Daten] [Peter Scambler, Brandon Wainwright, Michele Ramsay, Martin Farrall, Gill Bates, Meng-Fat Ho, Colin Cooper] Summary: The MET oncogene, present in the MNNG-HOS chemically transformed human cell line, is activated by a gene fusion involving sequences from chromosome 1 and chromosome 7. Activated MET can act as a dominant selectable marker for chromosome-mediated gene transfer, and several transfectant cell lines have been established using this technique. Analysis of the transgenomes within these cell lines indicates that MET activation is not simply due to a chromosome translocation, but may involve an interstitial insertion of DNA from chromosome 1, into chromosome 7, probably associated with other rearrangements. Pulse field gel analysis of two transfectants indicates that, despite the presence of complex rearrangements close to MET, chromosome 7 sequences are grossly intact over a 1-Mb region thought to contain the gene defective in cystic fibrosis. Springer-Verlag, 1990 Scambler Peter Department of Biochemistry and Molecular Genetics, St. Mary's Hospital Medical School, Norfolk Place, W2 1PG, London, UK aut Wainwright Brandon Department of Biochemistry and Molecular Genetics, St. Mary's Hospital Medical School, Norfolk Place, W2 1PG, London, UK aut Ramsay Michele Department of Biochemistry and Molecular Genetics, St. Mary's Hospital Medical School, Norfolk Place, W2 1PG, London, UK aut Farrall Martin Department of Biochemistry and Molecular Genetics, St. Mary's Hospital Medical School, Norfolk Place, W2 1PG, London, UK aut Bates Gill Department of Genome Analysis, ICRF, Lincoln's Inn Fields, WC2A 3PX, London, UK aut Ho Meng-Fat Department of Biochemistry and Molecular Genetics, St. Mary's Hospital Medical School, Norfolk Place, W2 1PG, London, UK aut Cooper Colin Section of Chemical Carcinogenesis, Institute of Cancer Research, Chester Beatty Laboratories, Fulham Road, SW36JB, London, UK aut Human Genetics Springer-Verlag 84/3(1990-02-01), 274-278 0340-6717 84:3<274 1990 84 439 https://doi.org/10.1007/BF00200574 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00200574 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Scambler Peter Department of Biochemistry and Molecular Genetics, St. Mary's Hospital Medical School, Norfolk Place, W2 1PG, London, UK aut NATIONALLICENCE 700 1- Wainwright Brandon Department of Biochemistry and Molecular Genetics, St. Mary's Hospital Medical School, Norfolk Place, W2 1PG, London, UK aut NATIONALLICENCE 700 1- Ramsay Michele Department of Biochemistry and Molecular Genetics, St. Mary's Hospital Medical School, Norfolk Place, W2 1PG, London, UK aut NATIONALLICENCE 700 1- Farrall Martin Department of Biochemistry and Molecular Genetics, St. Mary's Hospital Medical School, Norfolk Place, W2 1PG, London, UK aut NATIONALLICENCE 700 1- Bates Gill Department of Genome Analysis, ICRF, Lincoln's Inn Fields, WC2A 3PX, London, UK aut NATIONALLICENCE 700 1- Ho Meng-Fat Department of Biochemistry and Molecular Genetics, St. Mary's Hospital Medical School, Norfolk Place, W2 1PG, London, UK aut NATIONALLICENCE 700 1- Cooper Colin Section of Chemical Carcinogenesis, Institute of Cancer Research, Chester Beatty Laboratories, Fulham Road, SW36JB, London, UK aut NATIONALLICENCE 773 0- Human Genetics Springer-Verlag 84/3(1990-02-01), 274-278 0340-6717 84:3<274 1990 84 439 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer