caa a22 4500 467983585 CHVBK 20180323112525.0 cr unu---uuuuu 170328e19900201xx s 000 0 eng 10.1007/BF00200566 doi (NATIONALLICENCE)springer-10.1007/BF00200566 Demonstration of chromosome replication by BrdU antibody technique and electron microscopy [Elektronische Daten] [W. Vogel, K. Mehnert, S. Pentz] Summary: We describe the use of different reporter groups in visualizing replication patterns on metaphase chromosomes after BrdU incorporation by the BrdU antibody technique for the electron microscope. There is an inverse correlation between the density of the label and the size of the reporter particles. This observation alludes to stereo problems interfering with the access of the labeled antibodies into the chromatin. The use of silver enhancement enables easy detection of 1-nm and 5-nm gold particles, which make replication patterns visible in the electron microscope as does the diaminobenzidine/ H2O2 reaction. Possible consequences for the demonstration of replication patterns and for nonradioactive DNA in situ hybridization are discussed. Springer-Verlag, 1990 Vogel W. Abteilung Klinische Genetik der Universität, Albert-Einstein-Allee 11, D-7900, Ulm, Germany aut Mehnert K. Abteilung Klinische Genetik der Universität, Albert-Einstein-Allee 11, D-7900, Ulm, Germany aut Pentz S. Abteilung Klinische Genetik der Universität, Albert-Einstein-Allee 11, D-7900, Ulm, Germany aut Human Genetics Springer-Verlag 84/3(1990-02-01), 237-240 0340-6717 84:3<237 1990 84 439 https://doi.org/10.1007/BF00200566 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00200566 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vogel W. Abteilung Klinische Genetik der Universität, Albert-Einstein-Allee 11, D-7900, Ulm, Germany aut NATIONALLICENCE 700 1- Mehnert K. Abteilung Klinische Genetik der Universität, Albert-Einstein-Allee 11, D-7900, Ulm, Germany aut NATIONALLICENCE 700 1- Pentz S. Abteilung Klinische Genetik der Universität, Albert-Einstein-Allee 11, D-7900, Ulm, Germany aut NATIONALLICENCE 773 0- Human Genetics Springer-Verlag 84/3(1990-02-01), 237-240 0340-6717 84:3<237 1990 84 439 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer