caa a22 4500 467983887 CHVBK 20180323112526.0 cr unu---uuuuu 170328e19900401xx s 000 0 eng 10.1007/BF00195812 doi (NATIONALLICENCE)springer-10.1007/BF00195812 Isolation and regional localisation of DNA sequences from a human chromosome 11-specific cosmid library [Elektronische Daten] [Roy Wadey, Peter Little, Jon Pritchard, John Cowell] Summary: A cosmid library has been prepared in the lorist-B vector from a mouse/human somatic cell hybrid containing region 11q23-11pter as the only human component. This chromosome region is stably maintained in the hybrid as a result of translocation onto one copy of mouse chromosome 13. Individual cosmids containing human DNA were isolated by their ability to hybridise with total human DNA, digested with either HindIII or EcoRI, and 33 individual unique sequences were identified. These fragments were then isolated and subcloned into the bluescribe plasmid vector. Regional localisation of these unique sequences was achieved using a panel of somatic cell hybrids containing different overlapping deletions of chromosome 11. The majority of the 33 mapped sequences derived from the long arm of chromosome 11. Two clones were located within the 11p13-p14 region, which is associated with a predisposition to Wilms' tumour. These probes supplement those already mapped to this chromosome and will assist in the generation of a detailed chromosome 11 linkage map. Springer-Verlag, 1990 Wadey Roy Imperial Cancer Research Fund Laboratory of Molecular Genetics in the Department of Haematology and Oncology, Institute of Child Health, 30 Guilford Street, WC1N 1EH, London, UK aut Little Peter Department of Biochemistry, Imperial College of Science, Technology and Medicine, Exhibition Road, SW7, London, UK aut Pritchard Jon Imperial Cancer Research Fund Laboratory of Molecular Genetics in the Department of Haematology and Oncology, Institute of Child Health, 30 Guilford Street, WC1N 1EH, London, UK aut Cowell John Imperial Cancer Research Fund Laboratory of Molecular Genetics in the Department of Haematology and Oncology, Institute of Child Health, 30 Guilford Street, WC1N 1EH, London, UK aut Human Genetics Springer-Verlag 84/5(1990-04-01), 417-423 0340-6717 84:5<417 1990 84 439 https://doi.org/10.1007/BF00195812 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00195812 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wadey Roy Imperial Cancer Research Fund Laboratory of Molecular Genetics in the Department of Haematology and Oncology, Institute of Child Health, 30 Guilford Street, WC1N 1EH, London, UK aut NATIONALLICENCE 700 1- Little Peter Department of Biochemistry, Imperial College of Science, Technology and Medicine, Exhibition Road, SW7, London, UK aut NATIONALLICENCE 700 1- Pritchard Jon Imperial Cancer Research Fund Laboratory of Molecular Genetics in the Department of Haematology and Oncology, Institute of Child Health, 30 Guilford Street, WC1N 1EH, London, UK aut NATIONALLICENCE 700 1- Cowell John Imperial Cancer Research Fund Laboratory of Molecular Genetics in the Department of Haematology and Oncology, Institute of Child Health, 30 Guilford Street, WC1N 1EH, London, UK aut NATIONALLICENCE 773 0- Human Genetics Springer-Verlag 84/5(1990-04-01), 417-423 0340-6717 84:5<417 1990 84 439 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer