caa a22 4500 467983933 CHVBK 20180323112526.0 cr unu---uuuuu 170328e19900401xx s 000 0 eng 10.1007/BF00195821 doi (NATIONALLICENCE)springer-10.1007/BF00195821 Isolation of a cDNA clone encoding a novel form of granzyme B from human NK cells and mapping to chromosome 14 [Elektronische Daten] [Carol Dahl, Fritz Bach, Wing Chan, Kay Huebner, Giandomenico Russo, Carlo Croce, Thomas Herfurth, J. Cairns] Summary: We have isolated cDNA clones from a human NK cell cDNA library that encode the serine protease granzyme B. Although the sequence of the entire coding region for the mature protein and the 3′ untranslated region of the clone are identical to other cDNA isolates of this gene obtained from human T cell cDNA libraries, the 5′ end of two clones is 103 bp longer than the previously described sequences and would encode a protein with a 54-amino-acid-long signal sequence. Experiments characterizing granzyme B mRNA suggest that transcripts that initiate at or before the 5′ end of these clones comprise a detectable but infrequent class of granzyme B transcripts in NK and T cells. We have mapped this gene to human chromosome 14 in the region 14q11→14q32, distal to the T cell receptor alpha locus and proximal to the immunoglobulin heavy chain locus. The chromosomal location of this gene, together with the previously described high sequence homology between this gene and the mouse CTLA1/ccp1 gene, make it likely that this is the human equivalent of the mouse CTLA 1/ ccp1. Springer-Verlag, 1990 Dahl Carol Department of Pathology and Pittsburgh Cancer Institute, University of Pittsburgh, 3343 Forbes Avenue, 15213, Pittsburgh, PA, USA aut Bach Fritz Immunobiology Research Center, University of Minnesota, Minneapolis, Minnesota, USA aut Chan Wing Department of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia, USA aut Huebner Kay Wistar Institute, Philadelphia, Pennsylvania, USA aut Russo Giandomenico Wistar Institute, Philadelphia, Pennsylvania, USA aut Croce Carlo Wistar Institute, Philadelphia, Pennsylvania, USA aut Herfurth Thomas Department of Pathology and Pittsburgh Cancer Institute, University of Pittsburgh, 3343 Forbes Avenue, 15213, Pittsburgh, PA, USA aut Cairns J. Department of Pathology and Pittsburgh Cancer Institute, University of Pittsburgh, 3343 Forbes Avenue, 15213, Pittsburgh, PA, USA aut Human Genetics Springer-Verlag 84/5(1990-04-01), 465-470 0340-6717 84:5<465 1990 84 439 https://doi.org/10.1007/BF00195821 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00195821 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dahl Carol Department of Pathology and Pittsburgh Cancer Institute, University of Pittsburgh, 3343 Forbes Avenue, 15213, Pittsburgh, PA, USA aut NATIONALLICENCE 700 1- Bach Fritz Immunobiology Research Center, University of Minnesota, Minneapolis, Minnesota, USA aut NATIONALLICENCE 700 1- Chan Wing Department of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia, USA aut NATIONALLICENCE 700 1- Huebner Kay Wistar Institute, Philadelphia, Pennsylvania, USA aut NATIONALLICENCE 700 1- Russo Giandomenico Wistar Institute, Philadelphia, Pennsylvania, USA aut NATIONALLICENCE 700 1- Croce Carlo Wistar Institute, Philadelphia, Pennsylvania, USA aut NATIONALLICENCE 700 1- Herfurth Thomas Department of Pathology and Pittsburgh Cancer Institute, University of Pittsburgh, 3343 Forbes Avenue, 15213, Pittsburgh, PA, USA aut NATIONALLICENCE 700 1- Cairns J. Department of Pathology and Pittsburgh Cancer Institute, University of Pittsburgh, 3343 Forbes Avenue, 15213, Pittsburgh, PA, USA aut NATIONALLICENCE 773 0- Human Genetics Springer-Verlag 84/5(1990-04-01), 465-470 0340-6717 84:5<465 1990 84 439 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer