caa a22 4500 467984166 CHVBK 20180323112527.0 cr unu---uuuuu 170328e19900101xx s 000 0 eng 10.1007/BF00208937 doi (NATIONALLICENCE)springer-10.1007/BF00208937 Extent and high frequency of a short conversion between the human Aγ and Gγ fetal globin genes [Elektronische Daten] [J. Starck, R. Bouhass, F. Morlé, J. Godet] Summary: Southern blotting and DNA sequencing after polymerase chain reaction (PCR) amplification provide evidence for the frequent occurrence (in 7 out of 24 chromosomes) of a short conversion Gγ→Aγ in the 3′ end of the human fetal Aγ globin gene. This short conversion is characterized by the presence, 3 nucleotides downstream from the termination codon of the Aγ gene, of the TCAC sequence that is normally present at the equivalent position at the 3′ end of the Gγ gene; it is therefore identical to a conversion already described. Interestingly, we have found that this conversion is associated with the presence of theHindIII polymorphic restriction site in the Aγ IVS2, occuppying an equivalent position in both the Gγ and Aγ genes. Our observations strengthen the hypothesis that the presence of the HindIII polymorphic restriction site in Aγ IVS2 and the presence of the sequence TCAC at the 3′ end of the Aγ gene might be the result of a single conversion event. Springer-Verlag, 1990 Starck J. Centre de Génétique Moléculaire et Cellulaire, C.N.R.S. UMR 106, Université de Lyon I, F-69622, Villeurbanne Cedex, France aut Bouhass R. Service de Pédiatrie, Centre Emir Abdelkader, Oran, Algérie aut Morlé F. Centre de Génétique Moléculaire et Cellulaire, C.N.R.S. UMR 106, Université de Lyon I, F-69622, Villeurbanne Cedex, France aut Godet J. Centre de Génétique Moléculaire et Cellulaire, C.N.R.S. UMR 106, Université de Lyon I, F-69622, Villeurbanne Cedex, France aut Human Genetics Springer-Verlag 84/2(1990-01-01), 179-184 0340-6717 84:2<179 1990 84 439 https://doi.org/10.1007/BF00208937 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00208937 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Starck J. Centre de Génétique Moléculaire et Cellulaire, C.N.R.S. UMR 106, Université de Lyon I, F-69622, Villeurbanne Cedex, France aut NATIONALLICENCE 700 1- Bouhass R. Service de Pédiatrie, Centre Emir Abdelkader, Oran, Algérie aut NATIONALLICENCE 700 1- Morlé F. Centre de Génétique Moléculaire et Cellulaire, C.N.R.S. UMR 106, Université de Lyon I, F-69622, Villeurbanne Cedex, France aut NATIONALLICENCE 700 1- Godet J. Centre de Génétique Moléculaire et Cellulaire, C.N.R.S. UMR 106, Université de Lyon I, F-69622, Villeurbanne Cedex, France aut NATIONALLICENCE 773 0- Human Genetics Springer-Verlag 84/2(1990-01-01), 179-184 0340-6717 84:2<179 1990 84 439 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer